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Volume 154, Issue 1

Multiple phenotypic alterations caused by a -type cytochrome maturation gene mutation in Free

Abstract

In some biogenesis of -type cytochromes depends on the products of the () genes, which encode inner-membrane proteins. Inactivation of some genes, in particular , has an impact on other processes as well, including siderophore production and utilization. Non-polar insertions were generated in the , , , and genes, and their impacts on different phenotypes were compared. Only in the case of the mutant was cytochrome production totally abrogated. The mutant, and to a lesser extent the mutant, showed a range of other phenotypic changes. The production of the siderophore pyoverdine was very low and growth under the condition of iron limitation was severely restricted, but production of the second siderophore, pyochelin, was increased. Interestingly, other traits were also strongly affected by the mutation, including the production of pyocyanin, swarming and twitching motility, and rhamnolipid production. The production of -acyl homoserine lactones or the quinolone signal (PQS) was, however, not affected in the and mutants. The mutant was also found to accumulate porphyrins, and catalase production was undetectable, consistent with the increased sensitivity to hydrogen peroxide. Finally, reduction in the content of [Fe–S] clusters was evidenced in both and mutants. Wild-type phenotypes were restored by complementation with a gene from ATCC 17400. In conclusion, we have demonstrated that CcmC is a key determinant for cytochrome biogenesis, pyoverdine maturation, and expression of some quorum sensing-regulated traits.

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Keyword(s): AHL, N-acylhomoserine lactone , CAA, casamino acids , CAS, chrome azurol , EDDHA, ethylenediaminedihydroxyphenylacetic acid , HHQ, 2-heptyl-4-quinolone , HSL, homoserine lactone , PQS, Pseudomonas quinolone signal and TMBZ, 3,3′,5,5′-tetramethylbenzidine
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2008-01-01
2024-04-18
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Multiple phenotypic alterations caused by a c-type cytochrome maturation ccmC gene mutation in Pseudomonas aeruginosa
Microbiology 154, 127 (2008); https://doi.org/10.1099/mic.0.2007/008268-0
/content/journal/micro/10.1099/mic.0.2007/008268-0
/content/journal/micro/10.1099/mic.0.2007/008268-0
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