1887

Abstract

is a marine bacterium bearing 11 copies of ribosomal operons. In some strains, such as RIMD2210633, the genome includes identical copies of 16S rRNA genes (). However, it is known that other strains of the species, such as strains ATCC 17802 and RIMD2210856, show conspicuous intragenomic heterogeneity. The extent and diversity of the heterogeneity in were studied in further detail by characterization of the copies in environmental isolates belonging to 21 different genotype groups. Thirteen of these groups showed intragenomic heterogeneity, containing altogether 16 sequences differing within a 25 bp segment of their . These sequences grouped into four clusters differing in at least four nucleotide sites. Some isolates contained alleles from up to three different clusters. Each segment sequence conserved the stem–loop characteristic of the 16S rRNA structure of this 25 bp sequence. The double-stranded stem sequence was quite variable, but almost every variation had a compensatory change to maintain seven to eight paired bases. Conversely, the single-strand loop sequence was conserved. The results may be explained as a consequence of recombination among evolving in different bacteria. The results suggest that intergenomic recombination is very high in and that it occurs solely among species. This high homologous intergenomic recombination could be an effective mechanism to maintain intragenomic cohesion, mediating the dispersal of the most abundant version among the 11 intragenomic loci.

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2007-08-01
2024-03-28
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