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Volume 154, Issue 3

Multiple functions of ergosterol in the fission yeast Free

Abstract

Sterols are a major class of membrane lipids in eukaryotes. In , sterol 24-C-methyltransferase (Erg6p), C-8 sterol isomerase (Erg2p), C-5 sterol desaturase (Erg31p, Erg32p), C-22 sterol desaturase (Erg5p) and C-24 (28) sterol reductase (Sts1p/Erg4p) have been predicted, but not yet determined, to catalyse a sequence of reactions from zymosterol to ergosterol. Disruption mutants of these genes were unable to synthesize ergosterol, and most were tolerant to the polyene drugs amphotericin B and nystatin. Disruption of or did not cause ergosterol deficiency or tolerance to polyene drugs, indicating that the two C-5 sterol desaturases have overlapping functions. GFP-tagged DRM (detergent-resistant membrane)-associated protein Pma1p localized to the plasma membrane in Δ mutants. DRM fractionation revealed that the association between Pma1-GFP and DRM was weakened in Δ but not in other mutants. Several GFP-tagged plasma membrane proteins were tested, and an amino acid permease homologue, SPBC359.03c, was found to mislocalize to intracellular punctate structures in the Δ mutants. These results indicate that these proteins are responsible for ergosterol biosynthesis in fission yeast, similar to the situation in . Furthermore, in fission yeast, ergosterol is important for plasma membrane structure and function and for localization of plasma membrane proteins.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CHX, cycloheximide , CPY, carboxypeptidase Y , DRM, detergent-resistant membrane , GFP, green fluorescent protein , LY, Lucifer Yellow CH and TX-100, Triton X-100
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2008-03-01
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Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe
Microbiology 154, 830 (2008); https://doi.org/10.1099/mic.0.2007/011155-0
/content/journal/micro/10.1099/mic.0.2007/011155-0
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