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Volume 154, Issue 1

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study Free

Abstract

promotes transcription of a large cluster of genes involved in ribosome biogenesis. During growth in shake flasks, a mutant deleted for shows a small size phenotype and a reduced growth rate. We characterized the behaviour of an Δ mutant compared to an isogenic reference strain growing in chemostat cultures at the same specific growth rate. By studying glucose (anaerobic)- and ethanol (aerobic)-limited cultures we focused specifically on nutrient-dependent effects. Major differences in the genome-wide transcriptional profiles were observed during glucose-limited growth. In particular, Sfp1 appeared to be involved in the control of ribosome biogenesis but not of ribosomal protein gene expression. Flow cytometric analyses revealed size defects for the mutant under both growth conditions. Our results suggest that Sfp1 plays a role in transcriptional and cell size control, operating at two different levels of the regulatory network linking growth, metabolism and cell size.

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  • Accepted:
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Keyword(s): BI, budding index , FC, fold change , FDR, false discovery rate , FSC, forward scattering , PI, propidium iodide , PNM, pyrimidine nucleotide metabolism , RiBi, ribosome biogenesis , RNA Pol I, RNA polymerase I , RP, ribosomal protein and TIF, translation initiation factor
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2008-01-01
2024-04-25
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Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study
Microbiology 154, 337 (2008); https://doi.org/10.1099/mic.0.2007/011767-0
/content/journal/micro/10.1099/mic.0.2007/011767-0
/content/journal/micro/10.1099/mic.0.2007/011767-0
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