The acidic, glutamine-rich Mpn474 protein of Mycoplasma pneumoniae is surface exposed and covers the complete cell Hegermann, Jan and Halbedel, Sven and Dumke, Roger and Regula, Jörg and Gabdoulline, Razif R. and Mayer, Frank and Stülke, Jörg and Herrmann, Richard,, 154, 1185-1192 (2008), doi = https://doi.org/10.1099/mic.0.2007/013342-0, publicationName = Microbiology Society, issn = 1350-0872, abstract= The protein Mpn474 encoded by the mpn474 gene of the human-pathogenic Mycoplasma pneumoniae contains 1033 amino acids and has an isoelectric point of 4.79, which is caused by the large excess of glutamic acid residues (11 %). Although the protein lacks recognizable export signals we showed by immuno-electron microscopy that Mpn474 is surface exposed, covering the cell completely. By combining cross-linking and careful treatment of the bacterial cells with Triton X-100, we found that this protein is weakly bound to the cell surface, while the true transmembrane protein Mpn141 (adhesin P1) is firmly attached under the same experimental conditions. A transposon mutant in the mpn474 gene, which has no obvious phenotype, served as negative control for the immunodetection., language=, type=