1887

Abstract

AtrA, a transcriptional activator for II-ORF4, encoding the pathway-specific transcriptional activator of the actinorhodin biosynthetic gene cluster in A3(2), has been shown to bind the region upstream from the promoter of , encoding the pathway-specific transcriptional activator of the streptomycin biosynthetic gene cluster in [Uguru (2005) , 131–150]. The orthologue () in was constitutively transcribed throughout growth from a promoter located about 250 nt upstream of the translational start codon, as determined by S1 nuclease mapping. DNase I footprinting showed that histidine-tagged AtrA-g bound an inverted repeat located upstream of at positions –117 to –142 relative to the transcriptional start point of as +1. This AtrA-g-binding site was between two AdpA-binding sites at approximately nucleotide positions –270 and –50. AdpA is a central transcriptional activator in the A-factor regulatory cascade and essential for the transcription of . AtrA-g and AdpA simultaneously bound the respective binding sites. In contrast to AdpA, AtrA-g was non-essential for transcription; an -disrupted strain produced streptomycin on routine agar media to the same extent as the wild-type strain. However, the -disrupted strain tended to produce a smaller amount of streptomycin than the wild-type strain under some conditions, for example, on Bennett agar containing 1 % maltose and on a minimal medium. Therefore, AtrA-g had a conditionally positive effect on streptomycin production, as a tuner, probably by enhancing the AdpA-dependent transcriptional activation of in a still unknown manner.

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2008-03-01
2024-04-19
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References

  1. Ando N., Ueda K., Horinouchi S. 1997; A Streptomyces griseus gene ( sgaA ) suppresses the growth disturbance caused by high osmolality and a high concentration of A-factor during early growth. Microbiology 143:2715–2723
    [Google Scholar]
  2. Bentley S. D., Chater K. F., Cerdeño-Tárraga A. M., Challis G. L., Thomson N. R., James K. D., Harris D. E., Quail M. A., Kieser H. other authors 2002; Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2. Nature 417:141–147
    [Google Scholar]
  3. Fernández-Moreno M. A., Caballero J. L., Hopwood D. A., Malpartida F. 1991; The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces . Cell 66:769–780
    [Google Scholar]
  4. Higashi T., Iwasaki Y., Ohnishi Y., Horinouchi S. 2007; A-factor and phosphate depletion signals are transmitted to the grixazone biosynthesis genes via the pathway-specific transcriptional activator GriR. J Bacteriol 189:3515–3524
    [Google Scholar]
  5. Hirano S., Kato J., Ohnishi Y., Horinouchi S. 2006; Control of the Streptomyces subtilisin inhibitor gene by AdpA in the A-factor regulatory cascade in Streptomyces griseus . J Bacteriol 188:6207–6216
    [Google Scholar]
  6. Hong B., Phornphisutthimas S., Tilley E., Baumberg S., McDowall K. J. 2007; Streptomycin production by Streptomyces griseus can be modulated by a mechanism not associated with change in the adpA component of the A-factor cascade. Biotechnol Lett 29:57–64
    [Google Scholar]
  7. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: The John Innes Foundation;
    [Google Scholar]
  8. Horinouchi S. 2002; A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus . Front Biosci 7:d2045–d2057
    [Google Scholar]
  9. Horinouchi S. 2007; Mining and polishing of the treasure trove in the bacterial genus Streptomyces . Biosci Biotechnol Biochem 71:283–299
    [Google Scholar]
  10. Horinouchi S., Beppu T. 2007; Hormonal control by A-factor of morphological development and secondary metabolism in Streptomyces . Proc Jpn Acad Ser B 83:277–295
    [Google Scholar]
  11. Horinouchi S., Kumada Y., Beppu T. 1984; Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organisms: cloning and characterization. J Bacteriol 158:481–487
    [Google Scholar]
  12. Kato J., Chi W.-J., Ohnishi Y., Hong S.-K., Horinouchi S. 2005; Transcriptional control by A-factor of two trypsin genes in Streptomyces griseus . J Bacteriol 187:286–295
    [Google Scholar]
  13. Kato J., Funa N., Watanabe H., Ohnishi Y., Horinouchi S. 2007; Biosynthesis of γ -butyrolactone autoregulators that switch on secondary metabolism and morphological development in Streptomyces . Proc Natl Acad Sci U S A 104:2378–2383
    [Google Scholar]
  14. Oh S. H., Chater K. F. 1997; Denaturation of circular or linear DNA facilitates targeted integrative transformation of Streptomyces coelicolor A3(2): possible relevance to other organisms. J Bacteriol 179:122–127
    [Google Scholar]
  15. Ohnishi Y., Kameyama S., Onaka H., Horinouchi S. 1999; The A-factor regulatory cascade leading to streptomycin biosynthesis in Streptomyces griseus : identification of a target gene of the A-factor receptor. Mol Microbiol 34:102–111
    [Google Scholar]
  16. Ohnishi Y., Furusho Y., Higashi T., Chun H.-K., Furihata K., Sakuda S., Horinouchi S. 2004; Structures of grixazone A and B, A-factor-dependent yellow pigments produced under phosphate depletion by Streptomyces griseus . J Antibiot 57:218–223
    [Google Scholar]
  17. Ohnishi Y., Yamazaki H., Kato J., Tomono A., Horinouchi S. 2005; AdpA, a central transcriptional regulator in the A-factor regulatory cascade that leads to morphological development and secondary metabolism in Streptomyces griseus . Biosci Biotechnol Biochem 69:431–439
    [Google Scholar]
  18. Retzlaff L., Distler J. 1995; The regulator of streptomycin gene expression, StrR, of Streptomyces griseus is a DNA binding activator protein with multiple recognition sites. Mol Microbiol 18:151–162
    [Google Scholar]
  19. Strohl W. R. 1992; Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res 20:961–974
    [Google Scholar]
  20. Tomono A., Tsai Y., Yamazaki H., Ohnishi Y., Horinouchi S. 2005; Transcriptional control by A-factor of strR , the pathway-specific transcriptional activator for streptomycin biosynthesis in Streptomyces griseus . J Bacteriol 187:5595–5604
    [Google Scholar]
  21. Uguru G. C., Stephens K. E., Stead J. A., Towle J. E., Baumberg S., McDowall K. J. 2005; Transcriptional activation of the pathway-specific regulator of the actinorhodin biosynthetic genes in Streptomyces coelicolor . Mol Microbiol 58:131–150
    [Google Scholar]
  22. Vujaklija D., Ueda K., Hong S.-K., Beppu T., Horinouchi S. 1991; Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus . Mol Gen Genet 229:119–128
    [Google Scholar]
  23. Vujaklija D., Horinouchi S., Beppu T. 1993; Detection of an A-factor-responsive protein that binds to the upstream activation sequence of strR , a regulatory gene for streptomycin biosynthesis in Streptomyces griseus . J Bacteriol 175:2652–2661
    [Google Scholar]
  24. Yamazaki H., Ohnishi Y., Horinouchi S. 2000; An A-factor-dependent extracytoplasmic function sigma factor ( σ AdsA) that is essential for morphological development in Streptomyces griseus . J Bacteriol 182:4596–4605
    [Google Scholar]
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