Escherichia coli heat-shock proteins IbpA/B are involved in resistance to oxidative stress induced by copper
Matuszewska, Ewelina and Kwiatkowska, Joanna and Kuczyńska-Wiśnik, Dorota and Laskowska, Ewa,, 154, 1739-1747 (2008),
doi = https://doi.org/10.1099/mic.0.2007/014696-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= The small heat-shock proteins IbpA/B are molecular chaperones that bind denatured proteins and facilitate their subsequent refolding by the ATP-dependent chaperones DnaK, DnaJ, GrpE and ClpB. In this report, we demonstrate that IbpA/B participate in the defence against copper-induced stress under aerobic conditions. In the presence of oxygen, ΔibpA/B cells exhibit increased sensitivity to copper ions and accumulate elevated amounts of oxidized proteins, while under oxygen depletion, the ΔibpA/B mutation has no effect on copper tolerance. This indicates that IbpA/B protect Escherichia coli cells from oxidative damage caused by copper. We show that AdhE, one of the proteins exposed to oxidation, is protected by IbpA/B against copper-mediated inactivation both in vivo and in vitro.,
language=,
type=