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Volume 154, Issue 4

is involved in cellular invasion and survival Free

Abstract

HtrA is a heat-stress protein that functions both as a chaperone and as a serine protease. HtrA has been shown in several organisms to be involved in responses to stressful environmental conditions and involvement of HtrA in virulence has been reported in pathogenic species. A mutant demonstrated no significant difference to the W83 parent strain when subjected to high temperature and pH values from 3 to 11. However, the mutant showed increased sensitivity to HO. Cell invasion assays indicated that the total interaction (adherence) with KB cells, human coronary artery endothelial cells and gingival epithelial cells (GEC) was the same for both the wild-type and the mutant. However, the mutant showed increased invasion in KB cells and GEC. Microarray experiments indicated that a total of 253 genes were differentially regulated in the mutant, including a group of stress-related genes, which might be responsible for the observed decreased resistance to HO. In animal experiments, a competition assay showed that the mutant did not survive as well as the wild-type. In another assay, fewer mice infected with the mutant died than mice infected with W83, suggesting that the gene is virulence-related. These data indicate that the gene in does not relate to stress conditions such as high temperature and pH, but rather to HO stress. The gene also appears to be important for virulence and survival in animal models.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): BAP, blood agar plate , GEC, gingival epithelial cells , HCAEC, human coronary artery endothelial cells and refRNA, reference RNA
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2008-04-01
2024-04-19
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Porphyromonas gingivalis htrA is involved in cellular invasion and in vivo survival
Microbiology 154, 1161 (2008); https://doi.org/10.1099/mic.0.2007/015131-0
/content/journal/micro/10.1099/mic.0.2007/015131-0
/content/journal/micro/10.1099/mic.0.2007/015131-0
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