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Volume 154, Issue 6

Regulation and symbiotic significance of nodulation outer proteins secretion in HH103 Free

Abstract

In this work we show that the HH103 gene is essential for the expression of the genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate for the first time, to our knowledge, that the box preceding is necessary for Nops secretion. TtsI is responsible for the transcriptional activation of , , and . We confirm that the HH103 gene is activated by NodD1 and repressed by NolR. In contrast, NodD2 is not involved in the regulation of expression. Despite the dependence of expression of both and on NodD1 and flavonoids, clear differences in the capacity of some flavonoids to activate these genes were found. The expression of the and genes was also sensitive to differences in the pH of the media. Secretion of Nops in the mutant could not be complemented with a DNA fragment containing the gene and its box, but it was restored when a plasmid harbouring the , and y4xK genes was transferred to the mutant strain. The symbiotic effect of Nops secretion was host-dependent but independent of the type of nodule formed by the host legume. Nops are beneficial in the symbiosis with and , and detrimental in the case of the tropical legume .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): LCO, lipochitin oligosaccharide , Nops, nodulation outer proteins , T3SS, type III secretion system and α, Statistical significance of the differences observed using the Mann–Whitney non-parametric test
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2008-06-01
2024-04-18
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Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103
Microbiology 154, 1825 (2008); https://doi.org/10.1099/mic.0.2007/016337-0
/content/journal/micro/10.1099/mic.0.2007/016337-0
/content/journal/micro/10.1099/mic.0.2007/016337-0
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