RT Journal Article SR Electronic(1) A1 Husnain, Seyyed I. A1 Thomas, Mark S.YR 2008 T1 Downregulation of the Escherichia coli guaB promoter by FIS JF Microbiology, VO 154 IS 6 SP 1729 OP 1738 DO https://doi.org/10.1099/mic.0.2008/016774-0 PB Microbiology Society, SN 1465-2080, AB The Escherichia coli guaB promoter (P guaB ) regulates transcription of two genes, guaB and guaA, that are required for the synthesis of guanosine 5′-monophosphate (GMP), a precursor for the synthesis of guanine nucleoside triphosphates. Transcription from P guaB increases as a function of increasing cellular growth rate, and this is referred to as growth rate-dependent control (GRDC). Here we investigated the role of the factor for inversion stimulation (FIS) in the regulation of this promoter. The results showed that there are three binding sites for FIS centred near positions −11, +8 and +29 relative to the guaB transcription start site. Binding of FIS to these sites results in repression of P guaB in vitro but not in vivo. Deletion of the fis gene results in increased P guaB activity in vivo, but GRDC of P guaB is maintained., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2008/016774-0