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Volume 154, Issue 6

Downregulation of the promoter by FIS Free

Abstract

The promoter (P) regulates transcription of two genes, and , that are required for the synthesis of guanosine 5′-monophosphate (GMP), a precursor for the synthesis of guanine nucleoside triphosphates. Transcription from P increases as a function of increasing cellular growth rate, and this is referred to as growth rate-dependent control (GRDC). Here we investigated the role of the factor for inversion stimulation (FIS) in the regulation of this promoter. The results showed that there are three binding sites for FIS centred near positions −11, +8 and +29 relative to the transcription start site. Binding of FIS to these sites results in repression of P but not . Deletion of the gene results in increased P activity , but GRDC of P is maintained.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CRP, cAMP receptor protein , EMSA, electromobility shift assay , FIS, factor for inversion stimulation , GRDC, growth rate-dependent control and RNAP, RNA polymerase
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2008-06-01
2024-04-20
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Downregulation of the Escherichia coli guaB promoter by FIS
Microbiology 154, 1729 (2008); https://doi.org/10.1099/mic.0.2008/016774-0
/content/journal/micro/10.1099/mic.0.2008/016774-0
/content/journal/micro/10.1099/mic.0.2008/016774-0
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