Mitochondrial involvement in aspirin-induced apoptosis in yeast
Sapienza, Karen and Bannister, William and Balzan, Rena,, 154, 2740-2747 (2008),
doi = https://doi.org/10.1099/mic.0.2008/017228-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= We have previously reported that aspirin induces apoptosis in manganese superoxide dismutase (MnSOD)-deficient Saccharomyces cerevisiae cells when cultivated on the non-fermentable carbon source ethanol. Here, we investigated the role of mitochondria in aspirin-induced apoptosis. We report that aspirin had an inhibitory effect on cellular respiration, and caused the release of most of the mitochondrial cytochrome c and a dramatic drop in the mitochondrial membrane potential (ΔΨm). Also, aspirin reduced the intracellular cytosolic pH in the MnSOD-deficient cells growing in ethanol medium, but this did not seem to be the initial trigger that committed these cells to aspirin-induced apoptosis. Furthermore, loss of ΔΨm was not required for aspirin-induced release of cytochrome c, since the initial release of cytochrome c occurred prior to the disruption of the ΔΨm. It is thus possible that cytochrome c release does not involve the early onset of the mitochondrial permeability transition, but only an alteration of the permeability of the outer mitochondrial membrane.,
language=,
type=