The Bacillus subtilis ABC transporter EcsAB influences intramembrane proteolysis through RasP
Heinrich, Janine and Lundén, Tuula and Kontinen, Vesa P. and Wiegert, Thomas,, 154, 1989-1997 (2008),
doi = https://doi.org/10.1099/mic.0.2008/018648-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= The Bacillus subtilis σ W regulon is induced by different stresses that most probably affect integrity of the cell envelope. The activity of the extracytoplasmic function (ECF) sigma factor σ W is modulated by the transmembrane anti-sigma factor RsiW, which undergoes stress-induced degradation in a process known as regulated intramembrane proteolysis, finally resulting in the release of σ W and the transcription of σ W-controlled genes. Mutations in the ecsA gene, which encodes an ATP binding cassette (ABC) of an ABC transporter of unknown function, block site-2 proteolysis of RsiW by the intramembrane cleaving protease RasP (YluC). In addition, degradation of the cell division protein FtsL, which represents a second RasP substrate, is blocked in an ecsA-negative strain. The defect in σ W induction of an ecsA-knockout strain could be partly suppressed by overproducing RasP. A B. subtilis rasP-knockout strain displayed the same pleiotropic phenotype as an ecsA knockout, namely defects in processing α-amylase, in competence development, and in formation of multicellular structures known as biofilms.,
language=,
type=