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Volume 149, Issue 1

Diet influences the ecology of lactic acid bacteria and along the digestive tract of cattle: neural networks and 16S rDNA Free

Abstract

In this manuscript, the authors have sought to gain a better understanding of the interactions between and lactic acid bacteria (LAB) isolated from Rogossa MRS agar along the digestive tract of grain- and forage-fed cattle. from cattle receiving a high-grain diet were more numerous (<0·05) than from the high-forage diet and the highest numbers were in the faeces. Isolates on Rogossa MRS agar were always greater in the high-grain diet (<0·05) and contained a significant number of LAB. A random set of Rogossa MRS agar colonies was selected and artificial neural networks were used to develop a relationship between colony description and species which was validated using sequence analysis (16S rDNA). The neural networks correctly predicted species in more than 80 % of cases and was composed, primarily, of , , , , and . In conjunction with statistical diversity indices, it was demonstrated that diversity in the high-fibre diet was always lower and was a consequence of the dominance of . In contrast, the diversity in the high-grain diet was greater (<0·05) and was a consequence of the decline in . These data demonstrate that there is a positive relationship between coliform and LAB isolates throughout the digestive tract of cattle, and diet is the major factor regulating bacterial composition.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ANN, artificial neural network , AR, acid resistant , EHEC, enterohaemorrhagic Escherichia coli and LAB, lactic acid bacteria
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2003-01-01
2024-04-19
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Diet influences the ecology of lactic acid bacteria and Escherichia coli along the digestive tract of cattle: neural networks and 16S rDNA
Microbiology 149, 57 (2003); https://doi.org/10.1099/mic.0.25685-0
/content/journal/micro/10.1099/mic.0.25685-0
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