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Volume 149, Issue 1

Expressed sequence tag (EST) analysis of two subspecies of reveals a plethora of secreted proteins with potential activity in insect hosts Free

Abstract

Expressed sequence tag (EST) libraries for , the causative agent of green muscardine disease, were developed from the broad host-range pathogen sf. and the specific grasshopper pathogen, sf. . Approximately 1700 5′ end sequences from each subspecies were generated from cDNA libraries representing fungi grown under conditions that maximize secretion of cuticle-degrading enzymes. Both subspecies had ESTs for virtually all pathogenicity-related genes cloned to date from , but many novel genes encoding potential virulence factors were also tagged. Enzymes with potential targets in the insect host included proteases, chitinases, phospholipases, lipases, esterases, phosphatases and enzymes producing toxic secondary metabolites. A diverse array of proteases composed 36 % of all sf. ESTs. Eighty percent of the ESTs that could be clustered into functional groups had significant matches (<10) in other ascomycete fungi. These included genes reported to have specific roles in pathogens with plant or vertebrate hosts. Many of the remaining ESTs had their best match among animal, plant and bacterial sequences. These include genes with plant and microbial counterparts that produce potent antimicrobials. The abundance of transcripts discovered for different functional groups varied between the two subspecies of in a manner consistent with ecological adaptations of the two pathogens. By hastening gene discovery this project has enhanced development of improved mycoinsecticides. In addition, the ESTs represent a significant contribution to the extensive database of sequences from ascomycetes that are saprophytes or plant and vertebrate pathogens. Comparative analyses of these sequences is providing important information about the biology and evolutionary history of this clade.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): EST, expressed sequence tag
SGM
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2003-01-01
2024-04-20
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Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts
Microbiology 149, 239 (2003); https://doi.org/10.1099/mic.0.25761-0
/content/journal/micro/10.1099/mic.0.25761-0
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