1887

Abstract

Ageing in budding yeast is not determined by chronological lifespan, but by the number of times an individual cell is capable of dividing, termed its replicative capacity. As cells age they are subject to characteristic cell surface changes. reproduces asexually by budding and as a consequence of this process both mother and daughter cell retain chitinous scar tissue at the point of cytokinesis. Daughter cells exhibit a frail structure known as the birth scar, while mother cells display a more persistent bud scar. The number of bud scars present on the cell surface is directly related to the number of times a cell has divided and thus constitutes a biomarker for replicative cell age. It has been proposed that the birth scar may be subject to stretching caused by expansion of the daughter cell; however, no previous analysis of the effect of cell age on birth or bud scar size has been reported. This paper provides evidence that scar tissue expands with the cell during growth. It is postulated that symmetrically arranged breaks in the bud scar allow these rigid chitinous structures to expand without compromising cellular integrity.

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2003-11-01
2024-03-29
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References

  1. Bacon J. S. D., Davidson E. D., Jones D., Taylor I. F. 1966; The location of chitin in the yeast cell wall. Biochem J 101:36c–38c
    [Google Scholar]
  2. Barker M. G., Smart K. A. 1996; Morphological changes associated with the cellular ageing of a brewing yeast strain. J Am Soc Brew Chem 54:121–126
    [Google Scholar]
  3. Bartholomew J. W., Mittwer T. 1953; Demonstration of yeast bud scars with the electron microscope. J Bacteriol 65:272–275
    [Google Scholar]
  4. Barton A. A. 1950; Some aspects of cell division in Saccharomyces cerevisiae. J Gen Microbiol 4:84–86
    [Google Scholar]
  5. Beran K., Holan Z., Baldrian J. 1972; The chitin-glucan complex in Saccharomyces cerevisiae. I. IR and X-ray observations. Folia Microbiol 17:322–330
    [Google Scholar]
  6. Bulawa C. E. 1993; Genetics and molecular biology of chitin synthesis in fungi. Annu Rev Microbiol 47:505–534
    [Google Scholar]
  7. Cabib E., Bowers B. 1971; Chitin and yeast budding: localization of chitin in yeast bud scars. J Biol Chem 246:152–259
    [Google Scholar]
  8. Cabib E., Roberts R. 1982; Synthesis of the yeast cell wall and its regulation. Annu Rev Biochem 51:763–793
    [Google Scholar]
  9. Cabib E., Ulane R., Bowers B. 1974; A molecular model for morphogenesis: the primary septum of yeast. Curr Top Cell Regul 8:1–32
    [Google Scholar]
  10. Cabib E., Roberts S. R., Bowers B. 1982; Synthesis of the yeast cell wall and its regulation. Annu Rev Biochem 51:763–793
    [Google Scholar]
  11. Cabib E., Drgon T., Drgonova J., Ford R. A., Kollar R. 1997; The yeast cell wall, a dynamic structure engaged in growth and morphogenesis. Biochem Soc Trans 25:200–204
    [Google Scholar]
  12. Carter B. L., Jagadish M. N. 1978; The relationship between cell size and cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res 112:15–24
    [Google Scholar]
  13. Chant J., Pringle J. R. 1995; Patterns of bud-site selection in the yeast Saccharomyces cerevisiae. J Cell Biol 129:751–765
    [Google Scholar]
  14. Cid V. J., Duran A., Rey F. D., Snyder M. P., Nombela C., Sanchez M. 1995; Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59:345–386
    [Google Scholar]
  15. Dickinson J. R. 1999; Life cycle and morphogenesis. In The Metabolism and Molecular Physiology of Saccharomyces cerevisiae pp  1–22 Edited by Dickinson J. R., Schweizer M. London: Taylor & Francis;
    [Google Scholar]
  16. Egilmez N. K., Jazwinski S. M. 1989; Evidence for the involvement of a cytoplasmic factor in the aging of the yeast Saccharomyces cerevisiae. J Bacteriol 171:37–42
    [Google Scholar]
  17. Egilmez N. K., Chen J. B., Jazwinski S. M. 1990; Preparation and partial characterisation of old yeast cells. J Gerontol 45:B9–B17
    [Google Scholar]
  18. Friefelder D. 1960; Bud position in Saccharomyces cerevisiae. J Bacteriol 80:567–568
    [Google Scholar]
  19. Futcher B. 1993; Analysis of the cell cycle in Saccharomyces cerevisiae. In The Cell Cycle: a Practical Approach pp  69–92 Edited by Fantes P., Brooks R. Oxford: IRL Press;
    [Google Scholar]
  20. Galitski T., Saldanha A. J., Styles C. A., Lander E. S., Fink G. R. 1999; Ploidy regulation of gene expression. Science 285:251–254
    [Google Scholar]
  21. Goldstein I. J., Poretz R. D. 1986 Isolation and chemical properties of lectins. In The Lectins: Properties, Functions and Applications in Biology and Medicine pp  103–115 Edited by Liener I. E., Sharon N., Goldstein I. J. Orlando, FL: Academic Press;
    [Google Scholar]
  22. Gooday G. W. 1993; Cell-envelope diversity and dynamics in yeasts and filamentous fungi. J Appl Bacteriol 74:S12–S20
    [Google Scholar]
  23. Hartwell L. H., Unger M. W. 1977; Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. J Cell Biol 75:422–435
    [Google Scholar]
  24. Hayashibe M., Abe N., Matsui M. 1977; Mode of increase in cell wall polysaccharides in synchronously growing Saccharomyces cerevisiae. Arch Microbiol 114:91–97
    [Google Scholar]
  25. Hayflick L. 1965; The limited in vitro lifespan of human diploid cell strains. Exp Cell Res 37:614–636
    [Google Scholar]
  26. Hayflick L., Moorhead P. S. 1961; The serial cultivation of human diploid cell strains. Exp Cell Res 25:585–621
    [Google Scholar]
  27. Holan Z., Pokorny V., Beran K., Gemperle A., Tuzar Z., Baldrian J. 1981; The glucan-chitin complex in Saccharomyces cerevisiae. V. Precise location of chitin and glucan in bud scar and their physico-chemical characterization. Arch Microbiol 130:312–318
    [Google Scholar]
  28. Horisberger M., Volanthen M. 1977; Location of mannan and chitin on thin sections of budding yeast with gold markers. Arch Microbiol 115:1–7
    [Google Scholar]
  29. Jazwinski S. M. 1990; An experimental system for the molecular analysis of the aging process: the budding yeast Saccharomyces cerevisiae. J Gerontol 45:B68–B74
    [Google Scholar]
  30. Jazwinski S. M. 1993; Genes of youth: genetics of aging in baker's yeast. ASM News 59:172–178
    [Google Scholar]
  31. Johnson B. F., Lu C. 1975; Morphometric analysis of yeast cells IV. Increase of the cylindrical diameter of Schizosaccharomyces pombe during the cell cycle. Exp Cell Res 95:154–158
    [Google Scholar]
  32. Kennedy B. K., Austriaco N. R., Guarente L. 1994; Daughter cells of Saccharomyces cerevisiae from old mothers display a reduced life span. J Cell Biol 127:1985–1993
    [Google Scholar]
  33. Klis F. M. 1994; Cell wall assembly in yeast. Yeast 10:851–869
    [Google Scholar]
  34. Kollar R., Petrakova E., Ashwell G., Robbins P. W., Cabib E. 1995; Architecture of the yeast cell wall – the linkage between chitin and β(1–3)-glucan. J Biol Chem 270:1170–1178
    [Google Scholar]
  35. Kuntzel H., Schultz A., Ehbrecht I. M. 1996; Cell cycle control and initiation of DNA replication in Saccharomyces cerevisiae. Biol Chem 377:481–487
    [Google Scholar]
  36. Lorincz A., Carter B. L. A. 1979; Control of cell size at bud initiation in Saccharomyces cerevisiae. J Gen Microbiol 113:287–295
    [Google Scholar]
  37. Molano J., Polacheck I., Duran A., Cabib E. 1979; An endochitinase from wheat germ. Activity on nascent and performed chitin. J Biol Chem 254:4901–4907
    [Google Scholar]
  38. Mortimer R. K., Johnston J. R. 1959; Life span of individual yeast cells. Nature 183:1751–1752
    [Google Scholar]
  39. Motizuki M., Tsurugi K. 1992; The effect of aging on protein synthesis in the yeast Saccharomyces cerevisiae. Mech Age Dev 64:235–245
    [Google Scholar]
  40. Muller I. 1971; Experiments on ageing in single cells of Saccharomyces cerevisiae. Arch Mikrobiol 77:20–25
    [Google Scholar]
  41. Northcote D. H., Horne R. W. 1952; The chemical composition and structure of the yeast cell wall. Biochem J 51:232–236
    [Google Scholar]
  42. Orlean P. 1997 Biogenesis of yeast wall and surface components. In Molecular and Cellular Biology of the Yeast Saccharomyces. Cell Cycle and Cell Biology pp  229–362 Edited by Pringle J., Broach J., Jones E. W. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  43. Osumi M. 1998; The ultrastructure of yeast: cell wall structure and formation. Micron 29:207–233
    [Google Scholar]
  44. Powell C. D., Van Zandycke S. M., Quain D. E., Smart K. A. 2000; Replicative ageing and senescence in Saccharomyces cerevisiae and the impact on brewing fermentations. Microbiology 146:1023–1034
    [Google Scholar]
  45. Pringle J. R., Hartwell L. H. 1981 The Saccharomyces cerevisiae cell cycle. In The Molecular Biology of the Yeast Saccharomyces pp  97–141 Edited by Strathern J., Jones E. W., Broach J. R. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  46. Pringle J. R., Preston R. A., Adams A. E. M., Stearns T., Drubin D. G., Haarer B. K., Jones E. W. 1989; Fluorescence microscopy methods for yeast. Methods Cell Biol 31:357–435
    [Google Scholar]
  47. Roberts R. L., Bowers B., Slater M. L., Cabib E. 1983; Chitin synthesis and localization in cell division cycle mutants of Saccharomyces cerevisiae. Mol Cell Biol 3:922–930
    [Google Scholar]
  48. Sburlati A., Cabib E. 1986; Chitin synthase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae. J Biol Chem 261:15147–15152
    [Google Scholar]
  49. Seichertova O., Beran K., Holan Z., Pokorny V. 1973; The chitin-glucan complex of Saccharomyces cerevisiae. II. Location of the complex in the encircling region of the bud scar. Folia Microbiol 18:207–211
    [Google Scholar]
  50. Shaw J. A., Mol P. C., Bowers B., Silverman S. J., Valdivieso M. H., Duran A., Cabib E. 1991; The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114:111–123
    [Google Scholar]
  51. Sinclair D. A., Mills K. A., Guarente L. 1998; Aging in Saccharomyces cerevisiae. Annu Rev Microbiol 52:533–560
    [Google Scholar]
  52. Sloat B. F., Pringle J. R. 1978; A mutant of yeast defective in cellular morphogenesis. Science 200:1171–1173
    [Google Scholar]
  53. Strieblova E. 1970; Study of scar formation in the life cycle of the heterothallic Saccharomyces cerevisiae. Can J Microbiol 16:827–831
    [Google Scholar]
  54. Valdivieso M. H., Mol P. C., Shaw J. A., Cabib E., Duran A. 1991; Cloning of CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae. J Cell Biol 114:101–109
    [Google Scholar]
  55. Valentin E., Herrero E., Rico H., Miragall F., Sentandreu R. 1987; Cell wall mannoproteins during the population growth phases in Saccharomyces cerevisiae. Arch Microbiol 148:88–94
    [Google Scholar]
  56. Wheals A. E. 1987; Biology of the cell cycle in yeasts. In The Yeasts vol. 1, 2nd edn. Edited by Rose A. H., Harrison J. S. London: Academic Press;
    [Google Scholar]
  57. Woldringh C. L., Huls P. G., Vischer N. O. E. 1993; Volume growth of daughter and parent cells during the cell cycle of Saccharomyces cerevisiae a/ α as determined by image cytometry. J Bacteriol 175:3174–3181
    [Google Scholar]
  58. Woldringh C. L., Fluiter K., Huls P. G. 1995; Production of senescent cells of Saccharomyces cerevisiae by centrifugal elutriation. Yeast 11:361–369
    [Google Scholar]
  59. Yamaoka F., Kagei Y., Tomita S., Kondo Y., Hirano S. 1989; The structure of chitin-glucan complex isolated from yeast bud scars. Agric Biol Chem 53:1255–1259
    [Google Scholar]
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