RT Journal Article SR Electronic(1) A1 de Chial, Magaly A1 Ghysels, Bart A1 Beatson, Scott A. A1 Geoffroy, Valérie A1 Meyer, Jean Marie A1 Pattery, Theresa A1 Baysse, Christine A1 Chablain, Patrice A1 Parsons, Yasmin N. A1 Winstanley, Craig A1 Cordwell, Stuart J. A1 Cornelis, PierreYR 2003 T1 Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa JF Microbiology, VO 149 IS 4 SP 821 OP 831 DO https://doi.org/10.1099/mic.0.26136-0 PB Microbiology Society, SN 1465-2080, AB Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA. So far, for P. aeruginosa, three different PVDs, differing in their peptide chain, have been described (types I–III), but only the FpvA receptor for type I is known. Two PVD-producing P. aeruginosa strains, one type II and one type III, were mutagenized by a mini-TnphoA3 transposon. In each case, one mutant unable to grow in the presence of the strong iron chelator ethylenediaminedihydroxyphenylacetic acid (EDDHA) and the cognate PVD was selected. The first mutant, which had an insertion in the pvdE gene, upstream of fpvA, was unable to take up type II PVD and showed resistance to pyocin S3, which is known to use type II FpvA as receptor. The second mutant was unable to take up type III PVD and had the transposon insertion in fpvA. Cosmid libraries of the respective type II and type III PVD wild-type strains were constructed and screened for clones restoring the capacity to grow in the presence of PVD. From the respective complementing genomic fragments, type II and type III fpvA sequences were determined. When in trans, type II and type III fpvA restored PVD production, uptake, growth in the presence of EDDHA and, in the case of type II fpvA, pyocin S3 sensitivity. Complementation of fpvA mutants obtained by allelic exchange was achieved by the presence of cognate fpvA in trans. All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAI has a TAT export sequence at its N-terminal end., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26136-0