RT Journal Article SR Electronic(1) A1 Desnoues, Nicole A1 Lin, Min A1 Guo, Xianwu A1 Ma, Luyan A1 Carreño-Lopez, Ricardo A1 Elmerich, ClaudineYR 2003 T1 Nitrogen fixation genetics and regulation in a Pseudomonas stutzeri strain associated with rice JF Microbiology, VO 149 IS 8 SP 2251 OP 2262 DO https://doi.org/10.1099/mic.0.26270-0 PB Microbiology Society, SN 1465-2080, AB The Pseudomonas stutzeri strain A1501 (formerly known as Alcaligenes faecalis) fixes nitrogen under microaerobic conditions in the free-living state and colonizes rice endophytically. The authors characterized a region in strain A1501, corresponding to most of the nif genes and the rnf genes, involved in electron transport to nitrogenase in Rhodobacter capsulatus. The region contained three groups of genes arranged in the same order as in Azotobacter vinelandii: (1) nifB fdx ORF3 nifQ ORF5 ORF6; (2) nifLA-rnfABCDGEF-nifY2/nafY; (3) ORF13 ORF12-nifHDK-nifTY ORF1 ORF2-nifEN. Unlike in A. vinelandii, where these genes are not contiguous on the chromosome, but broken into two regions of the genome, the genes characterized here in P. stutzeri are contiguous and present on a 30 kb region in the genome of this organism. Insertion mutagenesis confirmed that most of the nif and the rnf genes in A1501 were essential for nitrogen fixation. Using lacZ fusions it was found that nif and rnf gene expression was under the control of ntrBC, nifLA and rpoN and that the rnf gene products were involved in the regulation of the nitrogen fixation process., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26270-0