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Volume 149, Issue 7

Bacterial lactoferrin-binding protein A binds to both domains of the human lactoferrin C-lobe Free

Abstract

Pathogenic bacteria in the family express surface receptors to acquire iron from the mammalian iron-binding proteins. Transferrins and lactoferrins constitute a family of iron-binding proteins highly related in both sequence and structure, yet the bacterial receptors are able to distinguish between these proteins and uphold a strict binding specificity. In order to understand the molecular basis for this specificity, the interaction between human lactoferrin (hLf) and the lactoferrin-binding protein A (LbpA) from was studied. A periplasmic expression system was designed for the heterologous expression of LbpA, which enabled the investigation of its binding activity in the absence of lactoferrin-binding protein B (LbpB). To facilitate delineation of the LbpA-binding regions of hLf, chimeric proteins composed of hLf and bovine transferrin were made. Binding studies performed with the chimeric proteins and recombinant LbpA identified two binding regions within the C-terminus of hLf. Furthermore, native LbpA from and spp. bound the identical spectrum of hybrid proteins as the recombinant receptor, demonstrating a conserved binding interaction with the C-lobe of hLf.

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Keyword(s): bTf, bovine transferrin , hLf, human lactoferrin and HRP, horseradish peroxidase
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2003-07-01
2024-04-18
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Bacterial lactoferrin-binding protein A binds to both domains of the human lactoferrin C-lobe
Microbiology 149, 1729 (2003); https://doi.org/10.1099/mic.0.26281-0
/content/journal/micro/10.1099/mic.0.26281-0
/content/journal/micro/10.1099/mic.0.26281-0
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