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Volume 150, Issue 7

Positive effects of multiple genes on expression of the locus of enterocyte effacement genes and adherence of enterohaemorrhagic O157 : H7 to HEp-2 cells Free

Abstract

Enteropathogenic and enterohaemorrhagic (EPEC and EHEC, respectively) genomes contain a pathogenicity island, termed the locus of enterocyte effacement (LEE), which encodes genes involved in the formation of attaching and effacing lesions on epithelial cells. To elucidate the regulatory mechanism of the LEE genes in EHEC, an EHEC O157 genomic library was screened for clones which modulated expression of the LEE genes. From more than 5000 clones, a DNA fragment was obtained containing a homologue as a positive regulator for the LEE genes. In EPEC, is known to be part of the operon, along with and , located on the EPEC adherence factor plasmid, which is not found in EHEC. However, the complete genome sequence of EHEC O157 Sakai strain reveals that there are five -like sequences, but no and , on the chromosome. These five homologues were characterized, and it was found that three of the homologues (renamed omologue , and ) encoded 104 aa proteins, and when expressed on a multicopy plasmid enhanced the expression of LEE genes. In contrast, homologues encoding proteins of 89 and 90 aa, renamed and , respectively, had no significant effect. Deletion mutants of the genes were constructed, and the effect on the expression of LEE-encoded type III effector proteins, such as EspA, B and D, and adhesion phenotype to HEp-2 cells was examined. Deletion of or , but not , decreased the expression of Esp proteins and adhesion to HEp-2 cells. Such effects were more apparent with mutants carrying double deletions of / or /, suggesting that // are all necessary for full expression of the LEE genes and adhesion to HEp-2 cells. Further study demonstrated that the positive effect of // was caused by enhanced transcription of the LEE-encoded regulatory gene, . Introduction of a multicopy plasmid carrying each // gene significantly induced microcolony formation by EHEC O157 on HEp-2 cells. These results suggest that the genes are necessary for full virulence of EHEC O157.

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  • Accepted:
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Keyword(s): AE (lesion), attaching and effacing , EHEC, enterohaemorrhagic Escherichia coli , EPEC, enteropathogenic Escherichia coli and LEE, locus of enterocyte effacement
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2004-07-01
2024-04-19
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Positive effects of multiple pch genes on expression of the locus of enterocyte effacement genes and adherence of enterohaemorrhagic Escherichia coli O157 : H7 to HEp-2 cells
Microbiology 150, 2357 (2004); https://doi.org/10.1099/mic.0.27100-0
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