1887

Abstract

The gene is involved in the pathway-specific positive regulation of biosynthesis of the antitumour polyketide landomycin E in 1912. LndI was overexpressed in as a protein C-terminally fused to the intein-chitin-binding-domain tag and purified in a one-step column procedure. Results of LndI titration, DNA gel mobility-shift assays and promoter-probing experiments indicate that LndI is an autoregulatory DNA-binding protein that binds to its own gene promoter and to the promoter of the structural gene . Enhanced green fluorescent protein was used as a reporter to study the temporal and spatial pattern of transcription. Expression of started before cells entered mid-exponential phase and peak expression coincided with maximal accumulation of landomycin E and biomass. In solid-phase analysis, expression was evident in substrate mycelia but was absent from aerial hyphae and spores.

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2005-01-01
2024-03-28
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References

  1. Arias P., Fernández-Moreno M. A., Malpartida F. 1999; Characterization of the pathway-specific positive transcriptional regulator for actinorhodin biosynthesis in Streptomyces coelicolor A3(2) as a DNA-binding protein. J Bacteriol 181:6958–6968
    [Google Scholar]
  2. Bourn W. R., Babb B. 1995; Computer assisted identification and classification of streptomycete promoters. Nucleic Acids Res 23:3696–3703 [CrossRef]
    [Google Scholar]
  3. Chater K. F. 1993; Genetics of differentiation in Streptomyces . Annu Rev Microbiol 47:685–713 [CrossRef]
    [Google Scholar]
  4. Fedorenko V., Basiliya L., Pankevych K., Dubitska L., Ostash B., Luzhetskyy A., Gromyko O., Krugel H. 2000; Genetic control of antitumor antibiotics-polyketides by actinomycetes. Bull Inst Agr Microbiol 8:27–31 in Ukrainian
    [Google Scholar]
  5. Fernandez-Moreno M. A., Caballero J. L., Hopwood D. A., Malpartida F. 1991; The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces . Cell 66:769–780 [CrossRef]
    [Google Scholar]
  6. Kataoka M., Kosono S., Tsujimoto G. 1999; Spatial and temporal regulation of protein expression by bldA within a Streptomyces lividans colony. FEBS Lett 462:425–429 [CrossRef]
    [Google Scholar]
  7. Khetan A., Hu W.-S., Sherman D. H. 2000; Heterogeneous distribution of lysine 6-aminotransferase during cephamycin C biosynthesis in Streptomyces clavuligerus demonstrated using green fluorescent protein as a reporter. Microbiology 146:1869–1880
    [Google Scholar]
  8. Kieser T., Bibb M. J., Buttner J. M., Chater K. F., Hopwood D. A. 2000 Practical Streptomyces Genetics Norwich, UK: John Innes Foundation;
    [Google Scholar]
  9. Krohn K., Rohr J. 1997; Angucyclines: total syntheses, new structures and biosynthetic studies of an emerging new class of antibiotics. Top Curr Chem 188:127–195
    [Google Scholar]
  10. Kyung Y.-S., Hu W.-S., Sherman D. H. 2001; Analysis of temporal and spatial expression of the CcaR regulatory element in the cephamycin C biosynthetic pathway using green fluorescent protein. Mol Microbiol 40:530–541 [CrossRef]
    [Google Scholar]
  11. Luzhetskii A. N., Ostash B. E., Fedorenko V. A. 2001; Intergeneric conjugation Escherichia coli - Streptomyces globisporus 1912 with using of integrative plasmid pSET152 and its derivative. Genetika 37:1340–1347 in Russian
    [Google Scholar]
  12. Martinez-Hackert E., Stock A. M. 1997; The DNA-binding domain of OmpR: crystal structure of a winged helix transcription factor. Structure 5:109–124 [CrossRef]
    [Google Scholar]
  13. Narva K. E., Feitelson J. S. 1990; Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2). J Bacteriol 172:326–333
    [Google Scholar]
  14. Ostash B., Rebets Yu., Yuskevich V., Luzhetskyy A., Tkachenko V., Fedorenko V. 2003a; Targeted disruption of Streptomyces globisporus lndF and lndL cyclase genes involved in landomycin E biosynthesis. Folia Microbiol 48:484–488 [CrossRef]
    [Google Scholar]
  15. Ostash B., Rebets Yu., Samborskyy M., Salas J. A., Fedorenko V. 2003b; Sequencing and analysis of putative 3-d and 4-th ring cyclase gene lndF of Streptomyces globisporus 1912 landomycin E biosynthesis gene cluster. Visn L'viv Univ Ser Biol 32:84–91
    [Google Scholar]
  16. Pankevych K., Kruegel H., Fedorenko V. 2001; Cloning and sequencing of a putative positive transcription regulator gene of landomycin E biosynthetic gene cluster of Streptomyces globisporus 1912. Visn L'viv Univ Ser Biol 27:97–105
    [Google Scholar]
  17. Paradkar A. S., Aidoo K. A., Jensen S. E. 1998; A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus . Mol Microbiol 27:831–843 [CrossRef]
    [Google Scholar]
  18. Polishchuk L. V., Hanusevych I. I., Matseliukh B. P. 1996; The antitumor action of antibiotics produced by Streptomyces globisporus 1912 studied in a model of Guerin's carcinoma in rats. Mikrobiol Zhurn 58:55–58 in Ukrainian
    [Google Scholar]
  19. Prentki P., Krisch H. M. 1984; In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303–313 [CrossRef]
    [Google Scholar]
  20. Rebets Y., Ostash B., Luzhetskyy A., Hoffmeister D., Brana A., Mendez C., Salas J. A., Bechthold A., Fedorenko V. 2003; Production of landomycins in Streptomyces globisporus 1912 and S. cyanogenus S136 is regulated by genes encoding putative transcriptional activators. FEMS Microbiol Lett 222:149–153 [CrossRef]
    [Google Scholar]
  21. Rodriguez L., Aguirrezabalaga I., Allende N., Brana A., Mendez C., Salas J. A. 2002; Engineering deoxysugar biosynthetic pathways from antibiotic-producing microorganisms. A tool to produce novel glycosylated bioactive compounds. Chem Biol 9:721–729 [CrossRef]
    [Google Scholar]
  22. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Santamarta I., Rodríguez-García A., Pérez-Redondo R., Martín J. F., Liras P. 2002; CcaR is an autoregulatory protein that binds to the ccaR and cefD-cmcI promoters of the cephamycin C-clavulanic acid cluster in Streptomyces clavuligerus . J Bacteriol 184:3106–3113 [CrossRef]
    [Google Scholar]
  24. Stephanopoulos G. 2002; Metabolic engineering by genome shuffling. Nat Biotechnol 20:666–668 [CrossRef]
    [Google Scholar]
  25. Stutzman-Engwall K. J., Otten S. L., Hutchinson C. R. 1992; Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius . J Bacteriol 174:144–154
    [Google Scholar]
  26. Sun J., Kelemen G. H., Fernandez-Abalos J. M., Bibb M. J. 1999; Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2). Microbiology 145:2221–2227
    [Google Scholar]
  27. Tang L., Grimm A., Zhang Y.-X., Hutchinson C. R. 1996; Purification and characterization of the DNA-binding protein DnRI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius . Mol Microbiol 22:801–813 [CrossRef]
    [Google Scholar]
  28. Taylor J. D., Ackroyd A. J., Halford S. E. 1994; The gel shift assay for the analysis of DNA–protein interaction. In Methods in Molecular Biology. DNA–Protein Interactions pp 263–281 Edited by Kneale G. G. Totowa, NJ: Humana Press;
    [Google Scholar]
  29. Trefzer A., Pelzer S., Schimana J., Stockert S., Bihlmaier C., Fiedler H. P., Welzel K., Vente A., Bechthold A. 2002; Biosynthetic gene cluster of simocyclinone, a natural multihybrid antibiotic. Antimicrob Agents Chemother 46:1174–1182 [CrossRef]
    [Google Scholar]
  30. Westrich L., Domann S., Faust B., Bedford D., Hopwood D. A., Bechthold A. 1999; Cloning and characterization of a gene cluster from Streptomyces cyanogenus S136 probably involved in landomycin A biosynthesis. FEMS Microbiol Lett 170:381–387 [CrossRef]
    [Google Scholar]
  31. Wietzorreck A., Bibb M. 1997; A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA binding fold. Mol Microbiol 25:1181–1184 [CrossRef]
    [Google Scholar]
  32. Yang K., Han L., He J., Wang L., Vining L. C. 2001; A repressor-response regulator gene pair controlling jadomycin B production in Streptomyces venezuelae ISP5230. Gene 279:165–173 [CrossRef]
    [Google Scholar]
  33. Zotchev S., Haugan K., Sekurova O., Sletta H., Ellingsen T. E., Valla S. 2000; Identification of a gene cluster for antibacterial polyketide-derived antibiotic biosynthesis in the nystatin producer Streptomyces noursei ATCC 11455. Microbiology 146:611–619
    [Google Scholar]
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