1887

Abstract

The amplification of an internal fragment of the gene for histidine decarboxylase in showed the gene to be located on the bacterial chromosome. Reverse PCR was then used to amplify both it and its adjacent genes. The histidine decarboxylase cluster was found to be composed of four genes: (expressed in , the product of which is located in the membrane, suggesting it to be a histidine/histamine antiporter), (which encodes histidine decarboxylase), (of unknown function but co-transcribed as bicistronic mRNA together with ) and (the only copy of a gene encoding a histidyl-tRNA synthetase in ). The expression of depends on the histidine concentration of the growth medium, and it can be transcribed as monocistronic or polycistronic mRNA.

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2005-04-01
2024-04-20
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