1887

Microbiology Society logo

Volume 150, Issue 12

Phycobilisome rod mutants in sp. strain PCC6803 Free

Abstract

The phycobilisome is a large pigment-protein assembly that harvests light energy for photosynthesis. This supramolecular complex is composed of two main structures: a core substructure and peripheral rods. Linker polypeptides assemble phycobiliproteins within these structures and optimize light absorption and energy transfer. Mutations have been constructed in three rod-linker-coding genes located in the operon of sp. strain PCC6803. The gene encoding the 33 kDa linker is found to be epistatic to encoding the 30 kDa linker, indicating a specific role for each of these two linkers in rod growth. This corroborates studies on the sequential degradation of phycobilisomes upon nitrogen starvation. Three allelic mutants affecting revealed a polar effect of commonly used cassettes (, ) on the operon steady-state transcripts and an effect of rod linker availability on the amount of phycocyanin incorporated in the phycobilisome. This led to the proposal that regulation of rod length could occur through processing of transcripts upstream of the gene.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): AP, allophycocyanin , FNR, ferredoxin NADP+ reductase , Lx, linker polypeptide located at position x of the phycobilisome, where x can be R (rod), RC (rod core) or CM (core membrane), linkers with identical location are distinguished by a superscript just above the x indicating their molecular mass , PBS, phycobilisome and PC, phycocyanin
© SGM
Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.27498-0
2004-12-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/150/12/mic1504147.html?itemId=/content/journal/micro/10.1099/mic.0.27498-0&mimeType=html&fmt=ahah

References

  1. Aiba, H., Adhya, S. & de Crombrugghe, B.(1981). Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem 256, 11905–11910. [Google Scholar]
  2. Ajlani, G. & Vernotte, C.(1998). Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803. Plant Mol Biol 37, 577–580.[CrossRef] [Google Scholar]
  3. Ajlani, G., Vernotte, C., DiMagno, L. & Haselkorn, R.(1995). Phycobilisome core mutants of Synechocystis PCC 6803. Biochim Biophys Acta 1231, 189–196.[CrossRef] [Google Scholar]
  4. Allen, M. M.(1968). Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 4, 1–4. [Google Scholar]
  5. Allen, M. M. & Smith, A. J.(1969). Nitrogen chlorosis in blue-green algae. Arch Mikrobiol 69, 114–120.[CrossRef] [Google Scholar]
  6. Belknap, W. R. & Haselkorn, R.(1987). Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena. EMBO J 6, 871–884. [Google Scholar]
  7. Bhalerao, R. P. & Gustafsson, P.(1994). Factors influencing the phycobilisome rod composition of the cyanobacterium Synechococcus sp. PCC7942: effects of reduced phycocyanin content, lack of rod-linkers and over-expression of the rod-terminating linker. Physiol Plant 90, 187–197.[CrossRef] [Google Scholar]
  8. Bhalerao, R. P., Gillbro, T. & Gustafsson, P.(1991). Structure and energy transfer of the phycobilisome in a linker protein replacement mutant of cyanobacterium Synechococcus 7942. Biochim Biophys Acta 1060, 59–66.[CrossRef] [Google Scholar]
  9. Bhalerao, R. P., Lind, L. K., Persson, C. E. & Gustafsson, P.(1993). Cloning of the phycobilisome rod linker genes from the cyanobacterium Synechococcus sp. PCC 6301 and their inactivation in Synechococcus sp. PCC 7942. Mol Gen Genet 237, 89–96. [Google Scholar]
  10. Bruce, D., Brimble, S. & Bryant, D. A.(1989). State transitions in a phycobilisome-less mutant of the cyanobacterium Synechococcus sp. PCC 7002. Biochim Biophys Acta 974, 66–73.[CrossRef] [Google Scholar]
  11. Cai, Y. & Wolk, C. P.(1990). Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. J Bacteriol 172, 3138–3145. [Google Scholar]
  12. Collier, J. L. & Grossman, A. R.(1994). A small polypeptide triggers complete degradation of light-harvesting phycobiliproteins in nutrient-deprived cyanobacteria. EMBO J 13, 1039–1047. [Google Scholar]
  13. de Lorimier, R., Bryant, D. A. & Stevens, S. E., Jr(1990a). Genetic analysis of a 9 kDa phycocyanin-associated linker polypeptide. Biochim Biophys Acta 1019, 29–41.[CrossRef] [Google Scholar]
  14. de Lorimier, R., Guglielmi, G., Bryant, D. A. & Stevens, S. E., Jr(1990b). Structure and mutation of a gene encoding a Mr 33,000 phycocyanin-associated linker polypeptide. Arch Microbiol 153, 541–549.[CrossRef] [Google Scholar]
  15. de Marsac, N. T. & Cohen-Bazire, G.(1977). Molecular composition of cyanobacterial phycobilisomes. Proc Natl Acad Sci U S A 74, 1635–1639.[CrossRef] [Google Scholar]
  16. Elmorjani, K., Thomas, J.-C. & Sebban, P.(1986). Phycobilisomes of wild type and pigment mutants of the cyanobacterium Synechocystis PCC 6803. Arch Microbiol 146, 186–191.[CrossRef] [Google Scholar]
  17. Fling, S. P. & Gregerson, D. S.(1986). Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Anal Biochem 155, 83–88.[CrossRef] [Google Scholar]
  18. Garnier, F., Dubacq, J. P. & Thomas, J. C.(1994). Evidence for a transient association of new proteins with the Spirulina maxima phycobilisome in relation to light Intensity. Plant Physiol 106, 747–754. [Google Scholar]
  19. Glazer, A. N.(1988). Phycobilisomes. Methods Enzymol 167, 304–312. [Google Scholar]
  20. Glazer, A. N.(1989). Light guides. Directional energy transfer in a photosynthetic antenna. J Biol Chem 264, 1–4. [Google Scholar]
  21. Golden, J. W. & Wiest, D. R.(1988). Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science 242, 1421–1423.[CrossRef] [Google Scholar]
  22. Gomez-Lojero, C., Perez-Gomez, B., Shen, G., Schluchter, W. M. & Bryant, D. A.(2003). Interaction of ferredoxin : NADP+ oxidoreductase with phycobilisomes and phycobilisome substructures of the cyanobacterium Synechococcus sp. strain PCC 7002. Biochemistry 42, 13800–13811.[CrossRef] [Google Scholar]
  23. Imashimizu, M., Fujiwara, S., Tanigawa, R., Tanaka, K., Hirokawa, T., Nakajima, Y., Higo, J. & Tsuzuki, M.(2003). Thymine at −5 is crucial for cpc promoter activity of Synechocystis sp. strain PCC 6714. J Bacteriol 185, 6477–6480.[CrossRef] [Google Scholar]
  24. Lagarde, D., Beuf, L. & Vermaas, W.(2000). Increased production of zeaxanthin and other pigments by application of genetic engineering techniques to Synechocystis sp. strain PCC 6803. Appl Environ Microbiol 66, 64–72.[CrossRef] [Google Scholar]
  25. Lönneborg, A., Lind, L. K., Kalla, S. R., Gustafsson, P. & Öquist, G.(1985). Acclimation processes in the light-harvesting system of the cyanobacterium Anacystis nidulans following a light shift from white to red light. Plant Physiol 78, 110–114.[CrossRef] [Google Scholar]
  26. Luque, I., Ochoa De Alda, J. A., Richaud, C., Zabulon, G., Thomas, J. C. & Houmard, J.(2003). The NblAI protein from the filamentous cyanobacterium Tolypothrix PCC 7601: regulation of its expression and interactions with phycobilisome components. Mol Microbiol 50, 1043–1054.[CrossRef] [Google Scholar]
  27. Mohamed, A. & Jansson, C.(1989). Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol 13, 693–700.[CrossRef] [Google Scholar]
  28. Oka, A., Sugisaki, H. & Takanami, M.(1981). Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol 147, 217–226.[CrossRef] [Google Scholar]
  29. Prentki, P. & Krisch, H. M.(1984). In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29, 303–313.[CrossRef] [Google Scholar]
  30. Rauhut, R. & Klug, G.(1999). mRNA degradation in bacteria. FEMS Microbiol Rev 23, 353–370.[CrossRef] [Google Scholar]
  31. Richaud, C., Zabulon, G., Joder, A. & Thomas, J. C.(2001). Nitrogen or sulfur starvation differentially affects phycobilisome degradation and expression of the nblA gene in Synechocystis strain PCC 6803. J Bacteriol 183, 2989–2994.[CrossRef] [Google Scholar]
  32. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  33. Schluchter, W. M. & Bryant, D. A.(1992). Molecular characterization of ferredoxin-NADP+ oxidoreductase in cyanobacteria: cloning and sequence of the petH gene of Synechococcus sp. PCC 7002 and studies on the gene product. Biochemistry 31, 3092–3102.[CrossRef] [Google Scholar]
  34. Sidler, W. A.(1994). Phycobilisome and phycobiliprotein structures. In The Molecular Biology of Cyanobacteria, pp. 139–216. Edited by D. A. Bryant. Dordrecht: Kluwer.
  35. van Thor, J. J., Gruters, O. W., Matthijs, H. C. & Hellingwerf, K. J.(1999). Localization and function of ferredoxin : NADP(+) reductase bound to the phycobilisomes of Synechocystis. EMBO J 18, 4128–4136.[CrossRef] [Google Scholar]
  36. Vioque, A.(1992). Analysis of the gene encoding the RNA subunit of ribonuclease P from cyanobacteria. Nucleic Acids Res 20, 6331–6337.[CrossRef] [Google Scholar]
  37. Yamanaka, G. & Glazer, A. N.(1980). Dynamic aspects of phycobilisome structure. Arch Microbiol 124, 39–47.[CrossRef] [Google Scholar]
  38. Yamanaka, G., Glazer, A. N. & Williams, R. C.(1980). Molecular architecture of a light-harvesting antenna. Comparison of wild type and mutant Synechococcus 6301 phycobilisomes. J Biol Chem 255, 11104–11110. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.27498-0
Loading
Phycobilisome rod mutants in Synechocystis sp. strain PCC6803
Microbiology 150, 4147 (2004); https://doi.org/10.1099/mic.0.27498-0
/content/journal/micro/10.1099/mic.0.27498-0
/content/journal/micro/10.1099/mic.0.27498-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error