%0 Journal Article %A Strauch, Mark A. %A Ballar, Petek %A Rowshan, Austin J. %A Zoller, Katherine L. %T The DNA-binding specificity of the Bacillus anthracis AbrB protein %D 2005 %J Microbiology, %V 151 %N 6 %P 1751-1759 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.27803-0 %I Microbiology Society, %X The Bacillus subtilis AbrB protein is a DNA-binding global regulator of a plethora of functions that are expressed during the transition from exponential growth to stationary phase and under suboptimal growth conditions. AbrB orthologues have been identified in a variety of prokaryotic organisms, notably in all species of Bacillus, Clostridium and Listeria that have been examined. Based on amino acid sequence identity in the N-terminal domains of the orthologues from B. subtilis and Bacillus anthracis, it was predicted that the proteins might display identical DNA-binding specificities. The binding of purified B. anthracis AbrB (AbrBBA) and purified B. subtilis AbrB (AbrBBS) at DNA targets of B. subtilis, B. anthracis and a synthetic origin was compared. In all cases examined, DNA-binding specificity was identical as judged by DNase I footprinting. In B. subtilis cells, the B. anthracis promoters from the atxA and abrB genes were regulated by AbrBBS, and the B. subtilis promoter from the yxbB operon was regulated by AbrBBA. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.27803-0