1887

Abstract

A gene encoding a putative peptidoglycan hydrolase was identified by sequence similarity searching in the 630 genome sequence, and the corresponding protein, named Acd (autolysin of ) was expressed in . The deduced amino acid sequence of Acd shows a modular structure with two main domains: an N-terminal domain exhibiting repeated sequences and a C-terminal catalytic domain. The C-terminal domain exhibits sequence similarity with the glucosaminidase domains of Atl and LytD autolysins. Purified recombinant Acd produced in was confirmed to be a cell-wall hydrolase with lytic activity on the peptidoglycan of several Gram-positive bacteria, including . The hydrolytic specificity of Acd was studied by RP-HPLC analysis and MALDI-TOF MS using cell-wall extracts. Muropeptides generated by Acd hydrolysis demonstrated that Acd hydrolyses peptidoglycan bonds between -acetylglucosamine and -acetylmuramic acid, confirming that Acd is an -acetylglucosaminidase. The transcription of the gene increased during vegetative cellular growth of 630. The sequence of the gene appears highly conserved in strains. Regarding deduced amino acid sequences, the C-terminal domain with enzymic function appears to be the most conserved of the two main domains. Acd is the first known autolysin involved in peptidoglycan hydrolysis of .

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2005-07-01
2024-03-28
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