1887

Abstract

Comparative genome hybridization using the pan- microarray identified genes from the gonococcal genetic island (GGI) within strains of serogroups W-135, H, and Z. While some of these strains contain nearly all of the genes of the GGI, there are differences in the presence of some of these genes between the strains, including between those of the same serogroup. Attempts were then made to determine the location of the GGI in these meningococci. Sequencing of strain MS11 revealed that the GGI is a conjugative plasmid that can be chromosomally integrated at the sites near and can also be present in its circularized form. In , a site is present in this location and also serves as the point of chromosomal integration of the GGI in this species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.27925-0
2005-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/151/12/4005.html?itemId=/content/journal/micro/10.1099/mic.0.27925-0&mimeType=html&fmt=ahah

References

  1. Bonfield J. K., Beal K. F., Betts M. J., Staden R. 2002; Trev: a DNA trace editor and viewer. Bioinformatics 18:194–195 [CrossRef]
    [Google Scholar]
  2. Correia F. F., Inouye S., Inouye M. 1986; A 26-base-pair repetitive sequence specific for Neisseria gonorrhoeae and Neisseria meningitidis genomic DNA. J Bacteriol 167:1009–1015
    [Google Scholar]
  3. Correia F. F., Inouye S., Inouye M. 1988; A family of small repeated elements with some transposon-like properties in the genome of Neisseria gonorrhoeae . J Biol Chem 263:12194–12198
    [Google Scholar]
  4. Dillard J. P., Hamilton H. L. 2002; Sequence and mutational analysis of the gonococcal genetic island. In Abstracts of the Thirteenth International Pathogenic Neisseria Conference p 79 Edited by Caugant D. A., Wedege E. Oslo: Nordberg Aksidenstrykkeri AS;
    [Google Scholar]
  5. Dillard J. P., Seifert H. S. 1997; A peptidoglycan hydrolase similar to bacteriophage endolysins acts as an autolysin in Neisseria gonorrhoeae . Mol Microbiol 25:893–901 [CrossRef]
    [Google Scholar]
  6. Dillard J. P., Seifert H. S. 2001; A variable genetic island specific for Neisseria gonorrhoeae is involved in providing DNA for natural transformation and is found more often in disseminated infection isolates. Mol Microbiol 41:263–277 [CrossRef]
    [Google Scholar]
  7. Elwell L. P., Falkow S. 1977; Plasmids of the genus Neisseria . In The Gonococcus pp 138–154 Edited by Roberts R. B. New York: Wiley;
    [Google Scholar]
  8. Hamilton H. L., Domínguez N. M., Schwartz K. J., Hackett K. T., Dillard J. P. 2005; Neisseria gonorrhoeae secretes chromosomal DNA via a novel type IV secretion system. Mol Microbiol 55:1704–1721 [CrossRef]
    [Google Scholar]
  9. Kellogg D. S., Peacock W. L., Deacon W. E., Brown L., Pirkle C. I Jr. 1963; Neisseria gonorrhoeae . I. Virulence genetically linked to clonal variation. J Bacteriol 85:1274–1279
    [Google Scholar]
  10. McAllister C. F., Stephens D. S. 1993; Analysis in Neisseria meningitidis and other Neisseria species of genes homologous to the FKBP immunophilin family. Mol Microbiol 10:13–23 [CrossRef]
    [Google Scholar]
  11. McShan W. M., Williams R. P., Hull R. A. 1987; A recombinant molecule from a disseminating strain of Neisseria gonorrhoeae that confers serum bactericidal resistance. Infect Immun 55:3017–3022
    [Google Scholar]
  12. Ménard R., Sansonetti P., Parsot C. 1994; The secretion of the Shigella flexneri Ipa invasins is activated by epithelial cells and controlled by IpaB and IpaD. EMBO J 13:5293–5302
    [Google Scholar]
  13. Parkhill J., Achtman M., James K. D. 25 other authors 2000; Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature 404:502–506 [CrossRef]
    [Google Scholar]
  14. Saunders N. J., Snyder L. A. S. 2002; The minimal mobile element. Microbiology 148:3756–3760
    [Google Scholar]
  15. Saunders N. J., Boonmee P., Peden J. F., Jarvis S. A. 2005; Inter-species horizontal transfer resulting in core-genome and niche-adaptive variation within Helicobacter pylori . BMC Genomics 6:9 [CrossRef]
    [Google Scholar]
  16. Snyder L. A. S., Saunders N. J., Shafer W. M. 2001; A putatively phase variable gene ( dca ) required for natural competence in Neisseria gonorrhoeae but not Neisseria meningitidis is located within the division cell wall ( dcw ) gene cluster. J Bacteriol 183:1233–1241 [CrossRef]
    [Google Scholar]
  17. Snyder L. A. S., Davies J. K., Saunders N. J. 2004; Microarray genomotyping of key experimental strains of Neisseria gonorrhoeae reveals gene complement diversity and five new neisserial genes associated with Minimal Mobile Elements. BMC Genomics 5:23 [CrossRef]
    [Google Scholar]
  18. Tettelin H., Saunders N. J., Heidelberg J. 39 other authors 2000; Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287:1809–1815 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.27925-0
Loading
/content/journal/micro/10.1099/mic.0.27925-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error