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Volume 151, Issue 7

Overproduction, purification and characterization of FtmPT1, a brevianamide F prenyltransferase from Free

Abstract

A putative prenyltransferase gene, , was identified in the genome sequence of . was cloned and expressed in , and the protein FtmPT1 was purified to near homogeneity and characterized biochemically. This enzyme was found to catalyse the prenylation of --trp--Pro (brevianamide F) at the C-2 position of the indole nucleus. FtmPT1 is a soluble monomeric protein, which does not contain the usual prenyl diphosphate binding site (N/D)DXXD found in most prenyltransferases, and which does not require divalent metal ions for its enzymic activity. values for brevianamide F and dimethylallyl diphosphate were determined as 55 and 74 μM, respectively. The turnover number was 5·57 s. FtmPT1 showed a high substrate specificity towards dimethylallyl diphosphate, but accepted different tryptophan-containing cyclic dipeptides. Together with dimethylallyltryptophan synthase of ergot alkaloid biosynthesis, FtmPT1 belongs to a new group of prenyltransferases with aromatic substrates.

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Keyword(s): DMAPP, dimethylallyl diphosphate , DMATS, dimethylallyltryptophan synthase , EI, electron impact and FAB, fast-atom bombardment
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2005-07-01
2024-04-20
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Overproduction, purification and characterization of FtmPT1, a brevianamide F prenyltransferase from Aspergillus fumigatus
Microbiology 151, 2199 (2005); https://doi.org/10.1099/mic.0.27962-0
/content/journal/micro/10.1099/mic.0.27962-0
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