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Volume 151, Issue 9

Noncomplementing diploidy resulting from spontaneous zygogenesis in Free

Abstract

With the aim of understanding sexual reproduction and phenotypic expression, a novel type of mating recently discovered in was investigated. Termed spontaneous zygogenesis (or Z-mating), it differs from F-mediated conjugation. Its products proved phenotypically unstable, losing part of the phenotype for which they were selected. Inactivation of a parental chromosome in the zygote is strongly suggested by fluctuation tests, respreading experiments, analysis of reisolates, and segregation of non-viable cells detected by epifluorescence staining. Some phenotypically haploid subclones were interpreted as stable noncomplementing diploids carrying an inactivated co-replicating chromosome. Pedigree analysis indicated that the genetic composition of such cells consisted of parental genomes or one parental plus a recombinant genome. Inactivation of a chromosome carrying a prophage resulted in the disappearance of both the ability to produce phage particles and the immunity to superinfection. Phage production signalled transient reactivation of such a chromosome and constituted a sensitive test for stable noncomplementing diploidy. Chromosome inactivation thus appears to be a spontaneous event in bacteria.

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Keyword(s): Ap, ampicillin , ApR, β-lactamase encoding plasmid , GDA, Gratia–Deschuyteneer agar medium , LC/LCA, lactose-casein broth/agar , MA, minimal agar (variants with additives) , NA, nutrient agar , Ncd, noncomplementing diploid , Nx, nalidixic acid , Sm, streptomycin , Szp+, spontaneous zygogenesis promoting and Z-mating, mating promoted by Szp+ bacteria
SGM
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2005-09-01
2024-04-19
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Noncomplementing diploidy resulting from spontaneous zygogenesis in Escherichia coli
Microbiology 151, 2947 (2005); https://doi.org/10.1099/mic.0.28096-0
/content/journal/micro/10.1099/mic.0.28096-0
/content/journal/micro/10.1099/mic.0.28096-0
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