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Volume 151, Issue 9

Novel surface layer protein genes in associated with unusual insertion elements Free

Abstract

The surface layer (S-layer) protein genes of the uranium mining waste pile isolate JG-A12 and of its relative NCTC 9602 were analysed. The almost identical N-termini of the two S-layer proteins possess a unique structure, comprising three N-terminal S-layer homologous (SLH) domains. The central parts of the proteins share a high homology and are related to the S-layer proteins of CCM 2177 and P-1. In contrast, the C-terminal parts of the S-layer proteins of JG-A12 and NCTC 9602 differ significantly between each other. Surprisingly, the C-terminal part of the S-layer protein of JG-A12 shares a high identity with that of the S-layer protein of CCM 2177. In both JG-A12 and NCTC 9602 the chromosomal S-layer protein genes are followed by a newly identified putative insertion element comprising three ORFs, which encode a putative transposase, a putative integrase/recombinase and a putative protein containing a DNA binding helix–turn–helix motif, and the S-layer-protein-like gene copies (9602) or (JG-A12). Interestingly, both strains studied were found to contain an additional, plasmid-located and silent S-layer protein gene with the same sequence as and . The primary structures of the corresponding putative proteins are almost identical in both strains. The N-terminal and central parts of these S-layer proteins share a high identity with those of the chromosomally encoded functional S-layer proteins. Their C-terminal parts, however, differ significantly. These results strongly suggest that the S-layer protein genes have evolved via horizontal transfer of genetic information followed by DNA rearrangements mediated by mobile elements.

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Keyword(s): S-layer, surface layer and SLH, S-layer homologous
SGM
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2005-09-01
2024-04-24
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Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements
Microbiology 151, 2961 (2005); https://doi.org/10.1099/mic.0.28201-0
/content/journal/micro/10.1099/mic.0.28201-0
/content/journal/micro/10.1099/mic.0.28201-0
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