@article{mbs:/content/journal/micro/10.1099/mic.0.28431-0, author = "Lavigne, Rob and Noben, Jean-Paul and Hertveldt, Kirsten and Ceyssens, Pieter-Jan and Briers, Yves and Dumont, Debora and Roucourt, Bart and Krylov, Victor N. and Mesyanzhinov, Vadim V. and Robben, Johan and Volckaert, Guido", title = "The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV", journal= "Microbiology", year = "2006", volume = "152", number = "2", pages = "529-534", doi = "https://doi.org/10.1099/mic.0.28431-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28431-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "WSA, whole-phage shotgun analysis", abstract = "The structural proteome of ϕKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in ϕKMV and suggest subtle structural differences from phage T7.", }