The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV Lavigne, Rob and Noben, Jean-Paul and Hertveldt, Kirsten and Ceyssens, Pieter-Jan and Briers, Yves and Dumont, Debora and Roucourt, Bart and Krylov, Victor N. and Mesyanzhinov, Vadim V. and Robben, Johan and Volckaert, Guido,, 152, 529-534 (2006), doi = https://doi.org/10.1099/mic.0.28431-0, publicationName = Microbiology Society, issn = 1350-0872, abstract= The structural proteome of ϕKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in ϕKMV and suggest subtle structural differences from phage T7., language=, type=