A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture O'Connell, Catherine M. and Nicks, Kristy M.,, 152, 1601-1607 (2006), doi = https://doi.org/10.1099/mic.0.28658-0, publicationName = Microbiology Society, issn = 1350-0872, abstract= A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4–30 %). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent., language=, type=