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Volume 152, Issue 5

Biofilm formation by WCS365: a role for LapD Free

Abstract

A role for the outer-membrane-associated LapA protein in early biofilm formation by WCS365 has previously been shown. This paper reports that , a gene located adjacent to the gene, also plays a role in biofilm formation. A mutation in results in a conditional biofilm defect in a static assay – this biofilm phenotype is exacerbated when biofilm formation is assayed in a flow-cell system. Furthermore, a mutation shows a partial defect in the transition from reversible to irreversible attachment, consistent with an early role for the gene product in biofilm formation. LapD is shown to be localized to the inner membrane of . The data show decreased LapA associated with the cell surface, but no apparent change in cytoplasmic levels of this protein or transcription, in a mutant. A model is proposed wherein the role of LapD in biofilm formation is modulating the secretion of the LapA adhesin.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ABC, ATP-binding cassette , CAA, Casamino acids , EPSII, galactoglucan and qRT-PCR, quantitative RT-PCR
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2006-05-01
2024-04-18
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Biofilm formation by Pseudomonas fluorescens WCS365: a role for LapD
Microbiology 152, 1375 (2006); https://doi.org/10.1099/mic.0.28696-0
/content/journal/micro/10.1099/mic.0.28696-0
/content/journal/micro/10.1099/mic.0.28696-0
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