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Volume 152, Issue 7

Effect of AmtB homologues on the post-translational regulation of nitrogenase activity in response to ammonium and energy signals in Free

Abstract

The AmtB protein transports uncharged NH into the cell, but it also interacts with the nitrogen regulatory protein P, which in turn regulates a variety of proteins involved in nitrogen fixation and utilization. Three P homologues, GlnB, GlnK and GlnJ, have been identified in the photosynthetic bacterium , and they have roles in at least four overlapping and distinct functions, one of which is the post-translational regulation of nitrogenase activity. In , nitrogenase activity is tightly regulated in response to addition or energy depletion (shift to darkness), and this regulation is catalysed by the post-translational regulatory system encoded by . Two homologues, and , have been identified in , and they are linked with and , respectively. Mutants lacking AmtB are defective in their response to both addition and darkness, while mutants lacking AmtB show little effect on the regulation of nitrogenase activity. These responses to darkness and appear to involve different signal transduction pathways, and the poor response to darkness does not seem to be an indirect result of perturbation of internal pools of nitrogen. It is also shown that AmtB is necessary to sequester detectable amounts GlnJ to the cell membrane. These results suggest that some element of the AmtB-P regulatory system senses energy deprivation and a consistent model for the integration of nitrogen, carbon and energy signals by P is proposed. Other results demonstrate a degree of specificity in interaction of AmtB with the different P homologues in . Such interaction specificity might be important in explaining the way in which P proteins regulate processes involved in nitrogen acquisition and utilization.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Amt, ammonium/methylammonium transport , DRAG, dinitrogenase reductase activating glycohydrolase , DRAT, dinitrogenase reductase ADP-ribosyl transferase , Gm, gentamycin , GS, glutamine synthetase , Km, kanamycin , MSX, methionine sulfoximine , Sm, streptomycin , Tc, tetracycline and α-KG, 2-oxoglutarate
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2006-07-01
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Effect of AmtB homologues on the post-translational regulation of nitrogenase activity in response to ammonium and energy signals in Rhodospirillum rubrum
Microbiology 152, 2075 (2006); https://doi.org/10.1099/mic.0.28903-0
/content/journal/micro/10.1099/mic.0.28903-0
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