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Volume 152, Issue 9

and its homologue are functionally equivalent Free

Abstract

is is an essential gene involved in cell division. This paper shows that and its homologue in are functionally equivalent. The genes are syntenous, and share significant homology in both their coding and non-coding DNA sequences. Transcription site mapping showed that the two genes possess near-identical promoter elements, and they displayed comparable promoter strengths in a reporter gene assay. The two proteins show near identity in their C-terminus, and polyclonal antiserum to WhmD specifically cross-reacts with a ∼15 kDa band in lysates. Following overexpression of sense and anti-sense constructs in their cognate mycobacterial hosts, and transformants displayed a small-colony phenotype, exhibited filamentation, and showed a reduction in viability. These observations reveal that the two proteins are functionally homologous and that their intracellular concentration is critical for septation in mycobacteria. Colonies of overexpressing were spherical and glossy, suggesting a change in composition of the cell envelope. Filaments of the conditionally complemented mutant were non-acid-fast, also indicating changes in characteristics of surface lipids. transformants carrying a fusion showed a diffuse pattern of fluorescence, consistent with the putative role of WhmD as a regulator. These observations strongly suggest that is an essential gene and its protein product in all likelihood regulates the expression of genes involved in the cell division cascade.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): 5′-RACE, rapid amplification of cDNA ends and 5′UTR, 5′ untranslated region
SGM
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2006-09-01
2024-04-18
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Mycobacterium smegmatis whmD and its homologue Mycobacterium tuberculosis whiB2 are functionally equivalent
Microbiology 152, 2735 (2006); https://doi.org/10.1099/mic.0.28911-0
/content/journal/micro/10.1099/mic.0.28911-0
/content/journal/micro/10.1099/mic.0.28911-0
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