@article{mbs:/content/journal/micro/10.1099/mic.0.29134-0, author = "Wang, Zhijun and Yuan, Zhenghong and Xiang, Li and Shao, Junjie and Węgrzyn, Grzegorz", title = "tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I", journal= "Microbiology", year = "2006", volume = "152", number = "12", pages = "3467-3476", doi = "https://doi.org/10.1099/mic.0.29134-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.29134-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Effects of tRNAAla(UGC) and its derivative devoid of the 3′-ACCA motif [tRNAAla(UGC)ΔACCA] on the cleavage of the ColE1-like plasmid-derived RNA I were analysed in vivo and in vitro. In an amino-acid-starved relA mutant, in which uncharged tRNAs occur in large amounts, three products of specific cleavage of RNA I were observed, in contrast to an otherwise isogenic relA + host. Overexpression of tRNAAla(UGC), which under such conditions occurs in Escherichia coli mostly in an uncharged form, induced RNA I cleavage and resulted in an increase in ColE1-like plasmid DNA copy number. Such effects were not observed during overexpression of the 3′-ACCA-truncated tRNAAla(UGC). Moreover, tRNAAla(UGC), but not tRNAAla(UGC)ΔACCA, caused RNA I cleavage in vitro in the presence of MgCl2. These results strongly suggest that tRNA-dependent RNA I cleavage occurs in ColE1-like plasmid-bearing E. coli, and demonstrate that tRNAAla(UGC) participates in specific degradation of RNA I in vivo and in vitro. This reaction is dependent on the presence of the 3′-ACCA motif of tRNAAla(UGC).", }