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Volume 152, Issue 10

Comparative analysis of two-component signal transduction systems of , and Free

Abstract

Members of the group are ubiquitously present in the environment and can adapt to a wide range of environmental fluctuations. In bacteria, these adaptive responses are generally mediated by two-component signal transduction systems (TCSs), which consist of a histidine kinase (HK) and its cognate response regulator (RR). With the use of techniques, a complete set of HKs and RRs was recovered from eight completely sequenced group genomes. By applying a bidirectional best-hits method combined with gene neighbourhood analysis, a footprint of these proteins was made. Around 40 HK-RR gene pairs were detected in each member of the group. In addition, each member contained many HK and RR genes not encoded in pairs (‘orphans’). Classification of HKs and RRs based on their enzymic domains together with the analysis of two neighbour-joining trees of these domains revealed putative interaction partners for most of the ‘orphans’. Putative biological functions, including involvement in virulence and host–microbe interactions, were predicted for the group HKs and RRs by comparing them with those of and other micro-organisms. Remarkably, appeared to lack specific HKs and RRs and was found to contain many truncated, putatively non-functional, HK and RR genes. It is hypothesized that specialization of as a pathogen could have reduced the range of environmental stimuli to which it is exposed. This may have rendered some of its TCSs obsolete, ultimately resulting in the deletion of some HK and RR genes.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): HK, histidine kinase , HMM, hidden Markov model , NCBI, National Center for Biotechnology Information , NJ, neighbour-joining , RR, response regulator and TCS, two-component signal transduction system
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2006-10-01
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Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis
Microbiology 152, 3035 (2006); https://doi.org/10.1099/mic.0.29137-0
/content/journal/micro/10.1099/mic.0.29137-0
/content/journal/micro/10.1099/mic.0.29137-0
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