1887

Abstract

causes symptoms of acute inflammatory diarrhoea in man. interaction with epithelial cells elicits interleukin-8 (IL-8) production, and IL-8 recruits neutrophils to sites of infection. Cell culture models of bacterial interaction with epithelium are useful to define bacteria–host interaction and are used because it is thought they mimic the same bacteria–host cell interaction in the natural disease. This study looks at the ability of strains to elicit IL-8 production from a variety of cell lines previously used for investigating the interaction of with host cells. A spectrum of IL-8 responses was observed, with minimal IL-8 elicited from Caco-2 cells and more marked responses elicited from HeLa and T84 cells. These -infected cell line responses were compared to IL-8 production from -infected human colonic and ileal tissue. The -infected tissue elicited the highest IL-8 responses and the cytokine was manifested earlier compared to the infected cell lines.

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2006-12-01
2024-03-28
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References

  1. Al-Salloom F. S, Al Mahmeed A, Ismaeel A, Botta G. A, Bakhiet M. 2003; Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles. J Infect 47:217–224 [CrossRef]
    [Google Scholar]
  2. Bakhiet M, Al-Salloom F. S, Qareiballa A, Bindayna K, Farid I, Botta G. A. 2004; Induction of alpha and beta chemokines by intestinal epithelial cells stimulated with Campylobacter jejuni . J Infect 48:236–244 [CrossRef]
    [Google Scholar]
  3. Everest P. 2005; Campylobacter jejuni and intestinal inflammation. In Campylobacter, Molecular and Cellular Biology pp.  421–434 Edited by Ketley J., Konkel M. Norwich: Horizon Press;
    [Google Scholar]
  4. Everest P. H, Goossens H, Butzler J. P, Lloyd D, Knutton S, Ketley J. M, Williams P. H. 1992; Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli . J Med Microbiol 37:319–325 [CrossRef]
    [Google Scholar]
  5. Everest P. H, Goossens H, Sibbons P, Lloyd D, Knutton S, Leece R, Ketley J. M, Williams P. H. 1993a; Pathological changes in the rabbit ileal loop model caused by Campylobacter jejuni from human colitis. J Med Microbiol 38:316–321 [CrossRef]
    [Google Scholar]
  6. Everest P. H, Cole A. T, Hawkey C. J, Goossens H, Knutton S, Butzler J. P, Ketley J. M, Williams P. H. 1993b; Roles of leukotriene B4, prostaglandin E2 and cyclic AMP in Campylobacter jejuni -induced intestinal fluid secretion. Infect Immun 61:4885–4887
    [Google Scholar]
  7. Harvey P, Battle T, Leach S. 1999; Different invasion phenotypes of Campylobacter isolates in Caco-2 cell monolayers. J Med Microbiol 48:461–469 [CrossRef]
    [Google Scholar]
  8. Hickey T. E, Baqar S, Bourgeois A. L, Guerry P. 1999; Campylobacter jejuni stimulated secretion of interleukin-8 by INT-407 cells. Infect Immun 67:88–93
    [Google Scholar]
  9. Hickey T. E, McVeigh A. L, Scott D. A, Michielutti R. E, Bixby A, Carroll S. A, Bourgeois A. L, Guerry P. 2000; Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect Immun 68:6535–6541 [CrossRef]
    [Google Scholar]
  10. Hickey T. E, Majam G, Guerry P. 2005; Intracellular survival of Campylobacter jejuni in human monocytic cells and induction of apoptotic death by cytolethal distending toxin. Infect Immun 73:5194–5197 [CrossRef]
    [Google Scholar]
  11. Hu L, Hickey T. E. 2005; Campylobacter jejuni induces secretion of proinflammatory chemokines from human intestinal epithelial cells. Infect Immun 73:4437–4440 [CrossRef]
    [Google Scholar]
  12. Ketley J. M. 1997; Pathogenesis of enteric infection by Campylobacter . Microbiology 143:5–21 [CrossRef]
    [Google Scholar]
  13. Konkel M. E, Monteville M. R, Rivera-Amill V, Joens L. 2001; The pathogenesis of Campylobacter jejuni -mediated enteritis. Curr Issues Intest Microbiol 2:55–71
    [Google Scholar]
  14. Kopecko D. J, Hu L, Zaal K. J. M. 2001; Campylobacter jejuni – microtubule-dependent invasion. Trends Microbiol 9:389–396 [CrossRef]
    [Google Scholar]
  15. Loss R. W, Mangla J. C, Pereira M. 1980; Campylobacter colitis presenting as inflammatory bowel disease with segmental colonic ulcerations. Gastroenterology 79:138–140
    [Google Scholar]
  16. Malago J. J, Koninkx J. F, Tooten P. C, van Liere E. A, van Dijk J. E. 2005; Anti-inflammatory properties of heat shock protein 70 and butyrate on Salmonella -induced interleukin-8 secretion in enterocyte-like Caco-2 cells. Clin Exp Immunol 141:62–71 [CrossRef]
    [Google Scholar]
  17. Masters J. R. 2002; HeLa cells 50 years on: the good, the bad and the ugly. Nat Rev Cancer 2:315–319 [CrossRef]
    [Google Scholar]
  18. Mellits K. H, Mullen J, Wand M, Armbruster G, Patel A, Connerton P. L, Skelly M, Connerton I. F. 2002; Activation of the transcription factor NF- κ B by Campylobacter jejuni . Microbiology 148:2753–2763
    [Google Scholar]
  19. Parkhill J, Achtman M, James K. D. 25 other authors 2000; The genome sequence of the food borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403:665–668 [CrossRef]
    [Google Scholar]
  20. Price A. B, Dolby J. M, Dunscombe P. R, Stirling J. 1984; Detection of Campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea. J Clin Pathol 37:1007–1013 [CrossRef]
    [Google Scholar]
  21. Russell R. G, Blaser M, Sarmiento J. I, Fox J. 1989; Experimental Campylobacter jejuni infection in Macaca nemestrina . Infect Immun 57:1438–1444
    [Google Scholar]
  22. Russell R. G, O'Donnoghue M, DeTolla L. J, Blake D. C Jr, Zulty J. 1993; Early colonic damage and invasion of Campylobacter jejuni in experimentally challenged infant Macaca mulatta . J Infect Dis 168:210–215 [CrossRef]
    [Google Scholar]
  23. Sansonetti P. 2002; Host pathogen interactions: the seduction of molecular cross talk. Gut 50: Suppl 3III2–III8
    [Google Scholar]
  24. Skirrow M. 1986; Campylobacter enteritis. In Medical Microbiology vol. 4 Edited by Easmon C. S. F. New York: Academic Press;
    [Google Scholar]
  25. Skirrow M, Blaser M. 2000; Clinical aspects of Campylobacter infection. In Campylobacter pp.  68–88 Edited by Nachamkin I., Blaser M. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Strober W. 1998; Interactions between epithelial cells and immune cells in the intestine. Ann N Y Acad Sci 859:37–45 [CrossRef]
    [Google Scholar]
  27. Van Spreeuwel P, Duursma G. C, Meijer C. J, Bax R, Rosekrans P. C. M, Lindeman J. 1985; Campylobacter colitis : histological immunohistochemical and ultrastructural findings. Gut 26:945–951 [CrossRef]
    [Google Scholar]
  28. Wassenaar T. M, Blaser M. J. 1999; Pathophysiology of Campylobacter jejuni infections of humans. Microbes Infect 1:1023–1033 [CrossRef]
    [Google Scholar]
  29. Weglarz L, Dzierzewicz Z, Orchel A, Szczerba J, Jaworska-kik M, Wilczok T. 2003; Biological activity of Desulfovibrio desulfuricans lipopolysaccharides evaluated via interleukin-8 secretion by Caco-2 cells. Scand J Gastroenterol 38:73–79
    [Google Scholar]
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