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Volume 153, Issue 1

Role of the alternative sigma factors and in survival of serovar Typhimurium during starvation, refrigeration and osmotic shock Free

Abstract

The ability of serovar Typhimurium to survive environmental stress requires specific, coordinated, responses, which induce resistance to the stress condition. This study investigated the relative contribution of and , the sigma factors regulating extracytoplasmic and general stress response functions, respectively, to survival at low temperature and also in media of differing osmotic strength, conditions relevant to food preservation. To determine if low-temperature storage is a signal for - and -mediated survival, the ability of . Typhimurium , and / mutants to survive in a saline starvation-survival model at a refrigeration temperature (4.5 °C) was examined. Under these conditions, the mutant was significantly (<0.05) compromised compared to the parent and to an mutant. The double mutant in and displayed a cumulative defect in survival. In hyperosmotic environments (low ) containing 6 % NaCl and at refrigeration temperature, both sigma factors were important for maximum survival but played the dominant role. Analysis of the metabolic activity of starved populations at 4.5 and 37 °C revealed significantly (<0.001) elevated electron-transport system activity in mutants in and , indicating a role for - and -regulated genes in maintaining energy homeostasis. Together these data demonstrate that and are important for survival of . Typhimurium in conditions encountered during food processing and that the relative contribution of and is critically dependent on the precise nature of the stress.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ETS, electron-transport system and INT, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
SGM
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2007-01-01
2024-04-16
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Role of the alternative sigma factors σE and σS in survival of Salmonella enterica serovar Typhimurium during starvation, refrigeration and osmotic shock
Microbiology 153, 263 (2007); https://doi.org/10.1099/mic.0.29235-0
/content/journal/micro/10.1099/mic.0.29235-0
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