1887

Abstract

Eighty-eight isolates, most of them from the Collection of Bacterial Strains of the Institut Pasteur, Paris, were analysed for their pyoverdine-mediated iron incorporation system by different methods, including pyoverdine isoelectrofocusing analysis, pyoverdine-mediated growth stimulation, immunoblot detection of (ferri)pyoverdine outer-membrane receptor and pyoverdine-facilitated iron uptake. The same grouping of the strains was reached by each of these methods, resulting in the classification of the isolates, even those which were devoid of pyoverdine production, into three different siderophore types. Forty-two percent of the strains were identified with the type-strain ATCC 15692 (group I). 42% were identical with the second type-strain ATCC 27853 (group II) and 16% reacted identically with the clinical isolate Pa6, whose pyoverdine was recognized in this study to be identical in structure to the pyoverdine produced by a natural isolate, strain R. No new pyoverdine species was detected among these strains.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-1-35
1997-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/1/mic-143-1-35.html?itemId=/content/journal/micro/10.1099/00221287-143-1-35&mimeType=html&fmt=ahah

References

  1. Ali N.J., Kessel D., Miller R.F. 1995; Bronchopulmonary infection with Pseudomonas aeruginosa in patients infected with human immunodeficiency virus.. Genitourin Med 71:73–77
    [Google Scholar]
  2. Ankenbauer R., Sriyosachati S., Cox C.D. 1985; Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin.. J Bacteriol 167:7–11
    [Google Scholar]
  3. Briskot G., Taraz K., Budzikiewicz H. 1989; Pyoverdin-type siderophores from Pseudomonas aeruginosa. . Liebigs Ann Chem 1989:375–384
    [Google Scholar]
  4. Budzikiewicz H. 1993; Secondary metabolites from fluorescent pseudomonads.. FEMS Microbiol Rev 104:209–228
    [Google Scholar]
  5. Campa M., Bendinelli M., Friedman H. editors 1993 Pseudomonas aeruginosa as an Opportunistic Pathogen New York: Plenum Press;
    [Google Scholar]
  6. Cornelis P., Hohnadel D., Meyer J.-M. 1989a; Evidence for different pyoverdine-mediated iron uptake systems among Pseudomonas aeruginosa strains.. Infect Immun 57:3491–3497
    [Google Scholar]
  7. Cornelis P., Bouia A., Guyonvarch A., Kammerer B., Hubert J.-C. 1989b; Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa. . Mol Microbiol 3:421–428
    [Google Scholar]
  8. De Vos D., Lim A. Jr De Vos P., Sarniguet A., Kersters K., Cornells P. 1993; Detection of the outer membrane lipoprotein I and its gene in fluorescent and non-fluorescent pseudomonads: implications for taxonomy and diagnosis.. J Gen Microbiol 139:2215–2223
    [Google Scholar]
  9. Doring G. 1993; Pseudomonas aeruginosa lung infection in cystic fibrosis patients.. In Pseudomonas aeruginosa as an Opportunistic Pathogen pp. 245–273 Edited by Campa M., Bendinelli M., Friedman H. New York: Plenum Press;
    [Google Scholar]
  10. Elliott R.P. 1958; Some properties of pyoverdine, the water- soluble pigment of the Pseudomonas. . Appl Microbiol 6:241–246
    [Google Scholar]
  11. Fichtenbaum C.J., Woeltfe K.F., Powderly W.G. 1994; Serious Pseudomonas aeruginosa infections in patients infected with human immunodeficiency virus: a case control study.. Clin Infect Dis 19:417–422
    [Google Scholar]
  12. Fleiszig S.M.J., Zaidi T.S., Fletcher E.L., Preston M.J., Pier G.B. 1994; Pseudomonas aeruginosa invades corneal epithelial cells during experimental infection.. Infect Immun 62:3485–3493
    [Google Scholar]
  13. Gipp S., Hahn J., Taraz K., Budzikiewicz H. 1991; Zwei Pyoverdine aus Pseudomonas aeruginosa R.. Z Naturforsch 46c:534–541
    [Google Scholar]
  14. Holder I.A. 1993; Pseudomonas aeruginosa burn infections: pathogenesis and treatment.. In Pseudomonas aeruginosa as an Opportunistic Pathogen pp. 275–295 Edited by Campa M., Bendinelli M., Friedman H. New York: Plenum Press;
    [Google Scholar]
  15. King E.0., Ward M.K., Raney D.F. 1954; Two simple media for the demonstration of pyocyanin and fluorescein.. J Lab Clin Med 44:301–307
    [Google Scholar]
  16. Koedam N., Wittouck E., Gaballa A., Gillis A., Höfte M., Cornells P. 1994; Detection and differentiation of microbial siderophores by isoelectric focusing and chrome azurol S overlay.. BioMetals 7:287–291
    [Google Scholar]
  17. Konopka K., Bindereif A., Neilands J.B. 1982; Aerobactin- mediated utilization of transferrin iron.. Biochemistry 21:6503–6508
    [Google Scholar]
  18. Koster M., van de Vossenberg J., Leong J., Weisbeek P.J. 1993; Identification and characterization of the pupB gene encoding an inducible ferric-pseudobactin receptor of Pseudomonas putida WCS358.. Mol Microbiol 8:591–601
    [Google Scholar]
  19. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  20. Massad G., Arceneaux J.E.L., Byers B.R. 1991; Acquisition of iron from host sources by mesophilic Aeromonas species.. J Gen Microbiol 137:237–241
    [Google Scholar]
  21. Meitert E. 1965; Schema de lysotypie pour Pseudomonas aeruginosa. . Arch Roum Pathol Exp Microbiol 24:439–458
    [Google Scholar]
  22. Meitert E. 1978; The study of antigenic relationships between bacteriophages of the romanian lysotyping scheme for Pseudomonas aeruginosa. . Arch Roum Pathol Exp Microbiol 37:123–128
    [Google Scholar]
  23. Meyer J.-M., Abdallah M.A. 1978; The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physicochemical properties.. J Gen Microbiol 107:319–328
    [Google Scholar]
  24. Meyer J.-M., Hallá F., Hohnadel D., Lemanceau P., Ratefiarivelo H. 1987; Siderophores of Pseudomonas - biological properties.. In Iron Transport in Microbes, Plants and Animals pp. 188–205 Edited by Winkelmann G., van der Helm D., Neilands J. B. Weinheim: VCH Verlagsgesellschaft;
    [Google Scholar]
  25. Meyer J.-M., Hohnadel D., Kahn A., Cornells P. 1990; Pyoverdine-facilitated iron uptake in Pseudomonas aeruginosa: immunological characterization of the ferripyoverdine receptor.. Mol Microbiol 4:1401–1405
    [Google Scholar]
  26. Meyer J.-M., Neely A., Stintzi A., Georges C., Holder I.A. 1996; Pyoverdine is essential for virulence of Pseudomonas aeruginosa. . Infect Immun 64:518–523
    [Google Scholar]
  27. Pugsley A.P., Oudega B. 1987; Methods for studying colicins and their plasmids.. In Plasmids, a Practical Approach pp. 105–161 Edited by Hardy K. G. Oxford: IRL Press;
    [Google Scholar]
  28. Romling U., Fiedler B., Bosshammer J., Grothues D., Greipel J., von der Hart H., Tümmler B. 1994; Epidemiology of chronic Pseudomonas aeruginosa infections in cystic fibrosis.. J Infect Dis 170:1616–1621
    [Google Scholar]
  29. Schryvers A.B. 1988; Characterization of the human transferrin and lactoferrin receptors in Haemophilus influenzae. . Mol Microbiol 2:467–472
    [Google Scholar]
  30. Schryvers A.B., Morris L.J. 1988; Identification and characterization of the transferrin receptor from Neisseria gonorrhoeae. . Mol Microbiol 2:281–288
    [Google Scholar]
  31. Sriyosachati S., Cox C.D. 1986; Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa. . Infect Immun 52:885–891
    [Google Scholar]
  32. Stoebner J.A., Payne S.M. 1988; Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholerae. . Infect Immun 56:2891–2895
    [Google Scholar]
  33. Tappe R., Taraz K., Budzikiewicz H., Meyer J.-M., Lefevre J.-F. 1993; Structure elucidation of a pyoverdin produced by Pseudomonas aeruginosa ATCC 27853.. J Prakt Chem 335:83–87
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-1-35
Loading
/content/journal/micro/10.1099/00221287-143-1-35
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error