1887

Abstract

A restriction map of the 8 Mb linear chromosome of R6-501 was constructed for the enzymes I (13 fragments) and I (7 fragments). Linking clones for all 12 I sites and 5 of the 6 I sites were isolated. The chromosome has terminal inverted repeats of 550 kb, which are the longest yet reported for a species. The oxytetracycline gene cluster lies about 600 kb from one end, which might account for its frequent spontaneous amplification and deletion. Several other markers were localized on the chromosome ( and the operons, the attachment site for pSAM2 and prophages RP2 and RP3). Comparison of the conserved markers with the map of A3(2) suggested there are differences in genome organization between the two species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-5-1493
1997-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/5/mic-143-5-1493.html?itemId=/content/journal/micro/10.1099/00221287-143-5-1493&mimeType=html&fmt=ahah

References

  1. Ali-Dunkrah U., Kendall K., Cullum J. 1990; Spontaneous mutations in the galactose operons of Streptomyces coelicolor A3 (2) and Streptomyces lividans 66. J Basic Microbiol 30:307–312
    [Google Scholar]
  2. Bolivar F., Rodrigez R. L., Green P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Costa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  3. bullock W. O., Fernandez J. M., Short J. M. 1987; XL1-B: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 5:376–379
    [Google Scholar]
  4. Butler M. J., Friend E. J., Hunter I. S., Kaczmarek F. S., Sudgen D. A., Warren M. 1989; Molecular cloning of resistance gene and architecture of a linked gene cluster involved in the biosynthesis of tetracycline by Streptomyces rimosus. Mol Gen Genet 215:231–238
    [Google Scholar]
  5. Evans G. A., Lewis K., Rothenberg B. E. 1989; High efficiency vectors for cosmid microcloning and genomic analysis. Gene 79:9–20
    [Google Scholar]
  6. Gravius B., Bezmalinović T., Hranueli D., Cullum J. 1993; Genetic instability and strain degeneration in Streptomyces rimosus. Streptomyces rimosus. Appl Environ Microbiol 59:2220–2228
    [Google Scholar]
  7. Gravius B., Cullum J., Hranueli D. 1994a; High G + C-content DNA markers for pulsed-field gel electrophoresis. Biotechniques 16:52
    [Google Scholar]
  8. Gravius B., Glocker D., Pigac J., Pandža K., Hranueli D., Cullum H. 1994b; The 387 kb linear plasmid pPZG101 of Streptomyces rimosus and its interactions with the chromosome. Microbiology 140:2271–2277
    [Google Scholar]
  9. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985; Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: The John Innes Foundation..
    [Google Scholar]
  10. Hranueli D., Pigac J., Vešligaj M. 1979; Characterization and persistence of actinophage RP2 isolated from Streptomyces rimosus ATCC 10970. J Gen Microbiol 114:295–303
    [Google Scholar]
  11. Hranueli D., Pandza K., Biuković G., Gravius B., Cullum J. 1995; Interaction of linear plasmid with Streptomyces rimosus chromosome: evidence for the linearity of chromosomal DNA. Croat Chem Acta 68:581–588
    [Google Scholar]
  12. Kalkus J., Dǒrrie C., Fischer D., Reh M., Schlegel H. G. 1993; The giant linear plasmid pHG207 from Rhodococcus sp. encoding hydrogen auxotrophy: characterization of the plasmid and its termini. J Gen Microbiol 139:2055–2065
    [Google Scholar]
  13. Kieser H., Kieser T., Hopwood D. A. 1992; A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome. J Bacteriol 174:5496–5507
    [Google Scholar]
  14. Leblond P., Redenbach M., Cullum J. 1993; Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol 175:3422–3429
    [Google Scholar]
  15. Leblond P., Fischer G., Francou F.-X., Berger F., Guerineau M., Decaris B. 1996; The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA. Mol Microbiol 19:261–271
    [Google Scholar]
  16. Lezhava A., Mizukami T., Kajitani T., Kameoka D., Redenbach M., Shinkawa H., Nimi O., Kinashi H. 1995; Physical map of the linear chromosome of Streptomyces griseus. J Bacteriol 177:6492–6498
    [Google Scholar]
  17. Lin Y.-S., Kieser H. M., Hopwood D. A., Chen C. W. 1993; The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol 10:923–933
    [Google Scholar]
  18. Mazodier P., Thompson C., Boccard F. 1990; The chromosomal integration site of the Streptomyces element pSAM2 overlaps a putative tRNA gene conserved among actinomycetes. Mol Gen Genet 222:431–434
    [Google Scholar]
  19. Musialowski M. S., Flett F., Scott G. B., Hobbs G., Smith C. P., Oliver S. G. 1994; Functional evidence that the principal DNA replication origin of the Streptomyces coelicolor chromosome is close to the dnaA-gyrB region. J Bacteriol 176:5123–5125
    [Google Scholar]
  20. Nußbaumer B., Wohlleben W. 1994; Identification, isolation and sequencing of the recA gene of Streptomyces lividans TK24. FEMS Microbiol Lett 118:57–64
    [Google Scholar]
  21. Perić N. 1995; Izolacija cjelovite nakupine otc gena soja Streptomyces rimosus R6. MSc thesis, University of Zagreb..
    [Google Scholar]
  22. Pigac J., Aladevid M. 1979; Mapping of oxytetracycline genes in Streptomyces rimosus. Period Biol 81:575–582
    [Google Scholar]
  23. Poustka A., Lehrach H. 1986; Jumping libraries and linking libraries: the next generation of molecular tools in mammalian genetics. Trends Genet 2:174–179
    [Google Scholar]
  24. Pujić P. 1992; Struktura rrnF operona za ribosomske RNA iz bakterije Streptomyces rimosus. MSc thesis, University of Zagreb..
    [Google Scholar]
  25. Rauland U., Glocker I., Redenbach M., Cullum J. 1995; DNA amplifications and deletions in Streptomyces lividans 66 and the loss of one end of the linear chromosome. Mol Gen Genet 246:37–44
    [Google Scholar]
  26. Rausch H., Vešligaj M., Podta D., Biukovid G., Pigac J., Cullum J., Schmieger H., Hranueli D. 1993; The temperate phages RP2 and RP3 of Streptomyces rimosus. J Gen Microbiol 139:2517–2524
    [Google Scholar]
  27. Redenbach M., Flett F., Piendl W., Glocker I., Rauland U., Wafzig O., Kliem R., Leblond P., Cullum J. 1993; The Streptomyces lividans 66 chromosome contains a 1 Mb deleto-genic region flanked by two amplifiable regions. Mol Gen Genet 241:255–262
    [Google Scholar]
  28. Redenbach M., Kieser H. M., Denapaite D., Eichner A., Cullum J., Kinashi H., Hopwood D. A. 1996; A set of ordered cosmids and a detailed genetic and physical map of the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol 21:77–96
    [Google Scholar]
  29. Smokvina T., Boccard F., Pernodet J.-L., Friedmann A., Guérineau M. 1991; Functional analysis of the Streptomyces ambofaciens element pSAM2. Plasmid 25:40–52
    [Google Scholar]
  30. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-5-1493
Loading
/content/journal/micro/10.1099/00221287-143-5-1493
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error