1887

Abstract

Summary: Both alleles of the gene of , which encodes a protein with exoglucanase activity, were sequentially disrupted. Enzymic analysis of either cell extracts or culture supernatants of disrupted strains revealed that this gene is responsible for the major exoglucanase activity in , although residual exoglucanase activity could still be detected. null mutants showed similar growth rates in both rich and minimal liquid medium as compared to the wild-type strain, indicating that the enzyme is not essential for growth. In addition, no differences were observed between wild-type and null mutants with respect to their ability to undergo dimorphic transition. However, small but repeatable differences were found between the wild-type and the null mutant with respect to susceptibility to chitin and glucan synthesis inhibitors. Using a murine model of experimental infection, no significant differences in virulence were observed. The null strain is thus a suitable recipient for studying gene expression using the exoglucanase as a reporter gene.

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1997-09-01
2024-03-28
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