1887

Abstract

SUMMARY: The early secreted antigenic target 6 kDa protein (ESAT-6) is a potent T-cell protein antigen synthesized by Mycobacterium tuberculosis. Its corresponding gene (esat-6) is located in RD1, a 10 kb DNA region deleted in the attenuated tuberculosis vaccine strain Mycobacterium bowis BCG. The promoter region of M. tuberculosis esat-6 was cloned and characterized. A new gene, designated lhp and cotranscribed with esat-6, was identified. Moreover, computer searches in the M. tuberculosis genome identified 13 genes related to the lhplesat-6 operon, defining a novel gene family. The transcription initiation sites of the lhplesat-6 operon were mapped using M. tuberculosis RNA. The corresponding promoter signals were not recognized in Mycobacterium smegmatis, in whichtranscription of lhplesat-6 is initiated at different locations. The M. tuberculosis lhp gene product was identified as CFP-10, a low- molecular-mass protein found in the short-term culture filtrate. These results show that the genes encoding CFP-10 and ESAT-6 are transcribed together in M. tuberculosis and that both code for small exported proteins.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-11-3195
1998-11-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/11/mic-144-11-3195.html?itemId=/content/journal/micro/10.1099/00221287-144-11-3195&mimeType=html&fmt=ahah

References

  1. Andersen P., Askgaard D., Ljungqvist L, Bentzon W.M., Heron I. 1991; T-cell proliferative response to antigens secreted by Mycobacterium tuberculosis. . Infect Immun 59:1558–1563
    [Google Scholar]
  2. Andersen P., Andersen Å.B., Sørensen A.L., Nagai S. 1995; Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice.. J Immunol 154:3359–3372
    [Google Scholar]
  3. Bashyam M.D., Tyagi A.K. 1994; An efficient and high- yielding method for isolation of RNA from mycobacteria.. Biotechniques 17:834–836
    [Google Scholar]
  4. Bashyam M.D., Kaushal D., Dasgupta K.K., Tyagi A.K. 1996; A study of the mycobacterial transcriptional apparatus: identification of novel features in promoter elements.. J Bacteriol 178:4847–4853
    [Google Scholar]
  5. Berthet F.-X., Rauzier J., Lim E.M., Philipp W., Gicquel B., PortnoÏ D. 1995; Characterization of the Mycobacterium tuberculosis erp gene encoding a potential cell surface protein with repetitive structures.. Microbiology 141:2123–2130
    [Google Scholar]
  6. Cole S.T., Brosch R., Parkhill J. 38 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.. Nature 393:537–544
    [Google Scholar]
  7. Collado-Vides J., Magasanik B.andGralla, Gralla J.D. 1991; Control site location and transcriptional regulation in Escherichia coli. . Microbiol Rev 55:371–394
    [Google Scholar]
  8. Collins D.M. 1996; In search of tuberculosis virulence genes.. Trends Microbiol 4:426–430
    [Google Scholar]
  9. Collins D.M, Kawakami R.P, De Lisle G.W, Pascopella L, Bloom B. R., Jacobs W. jr 1995; Mutation of the principal sigma factor causes loss of virulence in a strain of the Mycobacterium tuberculosis complex. . Proc Nut1 Acad Sci USA 92:8036–8040
    [Google Scholar]
  10. Fenton M.J., Vermeulen M. W. 1996; Immunopathology of tuberculosis: roles of macrophages and monocytes.. Infect Immun 64:683–690
    [Google Scholar]
  11. Harboe M., Oettinger T., Gotten Wiker H., Rosenkrands I., Andersen P. 1996; Evidence for occurence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG.. Infect Immun 64:16–22
    [Google Scholar]
  12. Jackson M., Portnoi D., Catheline D., Dumail L., Rauzier J., Legrand P., Gicquel B. 1997; Mycobacterium tuberculosis Des protein: an immunodominant target for the humoral response of tuberculous patients.. Infect Immun 65:2883–2889
    [Google Scholar]
  13. Kenney T.J., Churchward G. 1996; Genetic analysis of the Mycobacterium smegmatis rpsL promoter.. J Bacteriol 178:3564–3571
    [Google Scholar]
  14. Kremer L., Baulard A., Estaquier J., Content J., Capron A., Locht C. 1995; Analysis of the Mycobacterium tuberculosis antigen 85A promoter region.. J Bacteriol 177:642–653
    [Google Scholar]
  15. Maheiras G.G., Sabo P.J., Hickey M.J., Devinder C.S., Stover C.K. 1996; Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. . J BacterioL 178:1274–1282
    [Google Scholar]
  16. Quinn F.D., Newmann G.W., King C.H. 1996; Virulence determinants of Mycobacterium tuberculosis. . In Tuberculosis pp. 131–156 Berlin T.Shinnik. Heidelberg:: Springer;
    [Google Scholar]
  17. Sambrook J., Fritsch E.F., Maniatis T. 1989; Molecular Cloning:. a Laboratory Manual, 2. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  18. Satish M., Esser R.E., Thoile J.E.R., Clark-Curtiss J.E. 1990; Identification and characterization of antigenic determinants of Mycobacterium leprae that react with antibodies in sera of leprosy patients.. Infect Immun 58:1327–1336
    [Google Scholar]
  19. Sauton B. 1912; Sur la nutrition minerale du bacille tuberculeux.. C R Acad Sci 155:860–861
    [Google Scholar]
  20. Snapper S.B., Melton R.E., Mustafa S., Kieser T., Jacobs W.R. Jr 1990; Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. . Mol Microbiol 4:1911–1919
    [Google Scholar]
  21. Sørensen A.L., Nagai S., Houen G., Andersen P., Andersen Å.B. 1995; Purification and characterization of a low-molecular- mass T-cell antigen secreted by Mycobacterium tuberculosis. . Infect Immun 63:1710–1717
    [Google Scholar]
  22. Timm J., Lim E.M., Gicquel B. 1994; Escherichia coli- mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series.. J Bacteriol 176:6749–6753
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-11-3195
Loading
/content/journal/micro/10.1099/00221287-144-11-3195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error