The recombinant antigen ASPND1r from Aspergillus nidulans is specifically recognized by sera from patients with aspergilloma

References

1. Banerjee B., Kurup V. P., Phadnis S., Greenberger P. A., Fink J. N. 1996; Molecular cloning and expression of a recombinant Aspergillus fumigatus protein Asp f II with significant immunoglobulin E reactivity in allergic bronchopulmonary aspergillosis.. J Lab Clin Med 127:253–262
   [Google Scholar]
2. Calera J. A., López-Medrano R., Ovejero M. C., Puente P., Leal F. 1994; Variability of Aspergillus nidulans antigens with media and time and temperature of growth.. Infect Immun 62:2322–2333
   [Google Scholar]
3. Calera J. A., Ovejero M. C., López-Medrano R., Segurado M., Puente P., Leal F. 1997; Characterization of the Aspergillus nidulans aspnd1 gene demonstrates that the ASPND1 antigen, which it encodes, and several A. fumigatus immunodominant antigens belong to the same family.. Infect Immun 65:1335–1344
   [Google Scholar]
4. Frangioni J. V., Neel B. G. 1993; Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins.. Anal Biochem 210:179–187
   [Google Scholar]
5. Frankel S., Sohn R., Leinwand L. 1991; The use of sarkosyl in generating soluble protein after bacterial expression.. Proc Natl Acad Sci USA 88:1192–1196
   [Google Scholar]
6. Hamilton A. J., Holdom M. D., Hay R. J. 1995; Specific recognition of purified Cu, Zn superoxide dismutase from Aspergillus fumigatus by immune human sera.. J Clin Microbiol 33:495
   [Google Scholar]
7. Hearn V. M. 1988 Serodiagnosis of aspergillosis.. In Aspergillus and Aspergillosis pp 43–71 Edited by Vanden Bossche H., Mackenzie D. W. R., Cauwenbergh G. New York: Plenum;
   [Google Scholar]
8. Holdom M. D., Hay R. J., Hamilton A. J. 1995; Purification, N-terminal amino acid sequence and partial characterization of a Cu, Zn superoxide dismutase from the pathogenic fungus Aspergillus fumigatus . Free Rad Res 22:519–531
   [Google Scholar]
9. Huynh T. V., Young R. A., Davis R. W. 1985 Construction and screening of cDNA libraries in λgt10 and λgt11.. In DNA Cloning: a Practical Approach vol I pp 76–77 Edited by Glover D. M. Oxford: IRL Press;
   [Google Scholar]
10. Johnson K. S., Harrison G. B. L., Lightowlers M. W., O’Hoy K. L., Cougle W. G., Dempster R. P., Lawrence S. B., Vinton J. G., Heath D. D., Rickard M. D. 1989; Vaccination against ovine cysticercosis using a defined recombinant antigen.. Nature 338:585–587
    [Google Scholar]
11. Kumar A., Reddy, L V. & Sochanik A. 1993; Isolation and characterization of a recombinant heat-shock protein of Aspergillus fumigatus . J Allergy Clin Immunol 91:1024–1029
    [Google Scholar]
12. Kurup V. P., Kumar A. 1991; Immunodiagnosis of Aspergillosis.. Clin Microbiol Rev 4:439–456
    [Google Scholar]
13. Kwon-Chung K. J., Bennett J. E. 1992 Aspergillosis.. In Medical Mycology pp 201–247 Philadelphia, PA: Lea & Febiger;
    [Google Scholar]
14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
15. Lin S. X., Song G. C., Ding L Y., Lin Z. N., Cai Z. H. 1993; The possibility of GST antigen from Chinese strain of Schistosoma japonicum for immunodiagnosis of schistosomiasis.. Southeast Asian J Trop Med Public Health 24:70–73
    [Google Scholar]
16. López-Medrano R., Ovejero M. C., Calera J. A., Puente P., Leal F. 1995a; Aspergillus fumigatus antigens.. Microbiology 141:2699–2704
    [Google Scholar]
17. López-Medrano R., Ovejero M. C., Calera J. A., Puente P., Leal F. 1995b; An immunodominant 90-kilodalton Aspergillus fumigatus antigen is the subunit of a catalase.. Infect Immun 63:4774–4780
    [Google Scholar]
18. López-Medrano R., Ovejero M. C., Calera J. A., Puente P., Leal F. 1996; Immunoblotting patterns in the serodiagnosis of aspergilloma: antibody response to the 90 kDa Aspergillus fumigatus antigen.. Eur J Clin Microbiol Infect Dis 15:146–152
    [Google Scholar]
19. Marston A. O. 1987 The purification of eukaryotic poly-peptides expressed in Escherichia coli . In DNA Cloning: a Practical Approach vol III pp 61–62 Edited by Glover D. M. Oxford: IRL Press;
    [Google Scholar]
20. Morrisey J. H. 1981; Silver stain for proteins in polyacrylamide gel: a modified procedure with enhanced uniform sensitivity.. Anal Biochem 117:307–310
    [Google Scholar]
21. Moser M., Crameri R., Menz G., Schneider T., Dudler T., Virchow C., Gmachl M., Blaser K., Suter M. 1992; Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity.. J Immunol 149:454–460
    [Google Scholar]
22. Moser M., Crameri R., Brust E., Suter M., Menz G. 1994; Diagnostic value of recombinant Aspergillus fumigatus allergen I/a for skin testing and serology.. J Allergy Clin Immunol 93:1–11
    [Google Scholar]
23. Ron D., Dressler H. 1992; pGSTag – a versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins.. Bio Techniques 13:866–869
    [Google Scholar]
24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
25. Sanger F., Nicklens S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
26. Timberlake W. E., Hamer J. E. 1986 Regulation of gene activity during conidiophore development in Aspergillus nidulans . In Genetic Engineering vol 8 pp 1–29 Edited by Setlow J. K., Hollaender A. New York: Plenum Press;
    [Google Scholar]