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Volume 144, Issue 6

Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133: glnB or the P protein may be essential Free

Abstract

Bacterial P proteins, encoded by genes, are central signalling molecules in nitrogen regulatory pathways and are modulated by post-translational modification in response to the cellular nitrogen status. The gene was cloned from the filamentous heterocyst-forming cyanobacterium strain ATCC 29133 (PCC 73102) by heterologous hybridization to a sp. strain PCC 7942 gene fragment. Expression of the cloned gene was verified by hybridization to total RNA and a single cross-reactive polypeptide was observed in immunoblots of extracts probed with anti- 7942 P antiserum. Modification of the purified P protein by a 7942 P kinase was observed, but modified forms of P were not detected in extracts of from a variety of incubation conditions. The gene could not be disrupted by targeted gene replacement unless a second copy of was provided suggesting that the gene or gene product is essential for growth under the conditions tested.

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Keyword(s): diazotrophic cyanobacterium , glnB gene , Nostoc punctiforme, and PII protein
© Society Society for General Microbiology, 1998
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1998-06-01
2024-04-20
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References

  1. Allen, M. B., Arnon, D. I. (1955); Studies on nitrogen-fixing blue- green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm.. Plant Physiology, 30,:366–372
     [Google Scholar]
  2. Atkinson, M. R., Kamberov, E. S., Weiss, R. L., Ninfa, A. J. (1994); Reversible uridylylation of the Escherichia coli Pn signal transduction protein regulates its ability to stimulate the dephosphorylation of the transcription factor nitrogen regulator I (NRI or NtrC).. / Biol Chem, 269,:28288–28293
     [Google Scholar]
  3. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G. et al. (1987) Current Protocols in Molecular Biology. New York:: Green Publishing Associates &C Wiley Interscience;
     [Google Scholar]
  4. Bueno, R., Pahel, G., Magasanik B. (1985); Role of glnB and glnD gene products in regulation of the glnALG operon of Escherichia coli.. Journal of Bacteriology, 164,:816–822
     [Google Scholar]
  5. Buikema, W. J., Haselkorn R. (1991); Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120.. Genes Devel, 5,:321–330
     [Google Scholar]
  6. Cai, Y., Wolk C. P. (1990); Use of a conditionally lethal gene in Anabaena sp. strain PCC, 7120 to select for double recombinants and to entrap insertion sequences.. Journal of Bacteriology, 172,:3138–3145
     [Google Scholar]
  7. Campbell, D., Houmard, J., Tandeau de Marsac N. (1993); Electron transport regulates cellular differentiation in the filamentous cyanobacterium Calothrix.. Plant Cell, 5,:451–463
     [Google Scholar]
  8. Campbell, E. L., Hagen, K. D., Cohen, M. F., Summers, M. L., Meeks, J. C. (1996); The devR gene product is characteristic of receivers of two-component regulatory systems and is essential for heterocyst development in the filamentous cyanobacterium Nostoc sp. strain ATCC 29133.. Journal of Bacteriology, 178,:2037–2043
     [Google Scholar]
  9. Carr, P. D., Cheah, E., Suffolk, P. M., Vasudevan, S. G., Dixon, N. E. et al. (1996); X-ray structure of the signal transduction protein Pn from Escherichia coli at T9 A.. Acta Crystallographica, 52,:93–104
     [Google Scholar]
  10. Cohen, M. F., Meeks J. C. (1997); A hormogonium regulating locus, hrmUA, of the cyanobacterium Nostoc punctiforme strain ATCC 29133 and its response to an extract of a symbiotic plant partner Anthoceros punctatus.. Mol Plant-Microbe Interact, 10,:280–289
     [Google Scholar]
  11. Cohen, M. F., Wallis, J. G., Campbell, E. L., Meeks J. C. (1994); Transposon mutagenesis of Nostoc sp. strain ATCC 29133, a filamentous cyanobacterium with multiple cellular differentiation alternatives.. Microbiology, 140,:3233–3240
     [Google Scholar]
  12. Flores, E., Herrero A. (1994) Assimilatory nitrogen metabolism and its regulation.. Edited by Bryant, D. A. The Molecular Biology of Cyanobacteria. Dordrecht:: Kluwer,;487–517
     [Google Scholar]
  13. Forchhammer, K., Hedler, A. (1997); Phosphoprotein Pn from cyanobacteria - analysis of functional conservation with the Pn signal-transduction protein from Escherichia coli.. European Journal of Biochemistry, 244,:869–875
     [Google Scholar]
  14. Forchhammer, K., Tandeau de Marsac N. (1994); The Pn protein in the cyanobacterium Synechococcus sp. strain PCC, 7942 is modified by serine phosphorylation and signals the cellular N-status.. Journal of Bacteriology, 176,:84–91
     [Google Scholar]
  15. Forchhammer, K., Tandeau de Marsac, N. (1995a); Functional analysis of the phosphoprotein Pn (glnB gene product) in the cyanobacterium Synechococcus sp.. strain PCC, 7942. ] Bacteriol 177,:2033–2040
     [Google Scholar]
  16. Forchhammer, K., Tandeau de Marsac, N. (1995b); Phosphorylation of the Pn protein (glnB gene product) in the cyanobacterium Synechococcus sp. strain PCC 7942: analysis of an in vitro kinase activity.. Journal of Bacteriology, 177,:5812–5817
     [Google Scholar]
  17. van Heeswijk, W. C., Segeman, B., Hoving, S., Molenaar, D., Kahn, D., Westerhoff H. V. (1995); An additional Pn in Escherichia coli: a new regulatory protein in the glutamine synthetase cascade.. Fems Microbiology Letters, 132,:153–157
     [Google Scholar]
  18. Irmler, A., Sanner, S., Dierks, H., Forchhammer K. (1997); Dephosphorylation of the phosphoprotein P-II in Synechococcus PCC 7942: identification of an ATP and 2-oxoglutarate-regulated phosphatase activity.. Molecular Microbiology, 26,:81–90
     [Google Scholar]
  19. Jiang, P., Zucker, P., Atkinson, M. R., Kamberov, E. S.,, Tirasophon, W., Chandran P., Schefke, B. R., Ninfa, A. J. (1997a); Structure/ function analysis of the Pn signal transduction protein of Escherichia coli: genetic separation of interactions with protein receptors.. ] Bacteriol, 179,:4342–4353
     [Google Scholar]
  20. Jiang, P., Zucker, P., Ninfa A. J. (1997b); Probing interactions of the homotrimeric Pn signal transduction protein with its receptors by use of Pn heterotrimers formed in vitro from wild-type and mutant subunits.. Journal of Bacteriology, 179,:4354–4360
     [Google Scholar]
  21. Johansson, M., Nordlund S. (1997); Uridylylation of the Pu protein in the photosynthetic bacterium Rhodospirillum rubrum.. Journal of Bacteriology, 179,:4190–4194
     [Google Scholar]
  22. Kaneko, T., Sato, S., Kotani, H. (1996); 8c 21 other authors, Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp.. strain PCC 6803, 3,:109–136
     [Google Scholar]
  23. Khyudakov, I., Wolk C. P. (1996); Evidence that the hanA gene coding for HU protein is essential for heterocyst differentiation in, and cyanophage A-4(L) sensitivity of, Anabaena sp. strain PCC 7120.. Journal of Bacteriology, 178,:3572–3577
     [Google Scholar]
  24. Liotenberg, S., Campbell, D., Castets, A. M., Houmard, J., Tandeau de Marsac N. (1996); Modification of the Pn protein in response to carbon and nitrogen availability in filamentous cyanobacteria.. Fems Microbiology Letters, 144,:185–190
     [Google Scholar]
  25. Michel-Reydellet, N., Desnoues, N., Elmerich, C., Kaminski, P. A. (1997); Characterization of Azorhizobium caulinodans glnB and glnA genes: involvement of the Pn protein in symbiotic nitrogen fixation.. Journal of Bacteriology, 179,:3580–3587
     [Google Scholar]
  26. Muro-Pastor, M. I., Florencio F. J. (1994); NADP+-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7120: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene.. Journal of Bacteriology, 176,:2718–2726
     [Google Scholar]
  27. Rippka, R., Herdman, M. (1992) Pasteur Culture Collection of Cyanobacteria in Axenic Culture. Paris:: Institut Pasteur;
     [Google Scholar]
  28. Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., Stanier R. Y. (1979); Generic assignments, strain histories and properties of pure cultures of cyanobacteria.. / Gen Microbiol, 111,:1–61
     [Google Scholar]
  29. Stewart, W. D. P., Rowell, P. (1975); Effects of L-methionine-DL- sulfoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica.. Biocbem Biophys Res Commun, 65,:846–856
     [Google Scholar]
  30. Summers, M. L., Wallis, J. G., Campbell, E. L., Meeks, J. C. (1995); Genetic evidence of a major role for glucose-6-phosphate dehydrogenase in nitrogen fixation and dark growth of the cyanobacterium Nostoc sp.. strain ATCC, 29133. / Bacteriol 177,:6184–6194
     [Google Scholar]
  31. Tandeau de Marsac, N. (1994) Differentiation of hormogonia and relationships with other biological processes.. Edited by Bryant, D. A. The Molecular Biology of Cyanobacteria. Dordrecht:: Kluwer,;825–842
     [Google Scholar]
  32. Tandeau de Marsac, N., Houmard, J. (1993); Adaptation of cyanobacteria to environmental stimuli — new steps towards molecular mechanisms.. Fems Microbiology Reviews, 104,:119–189
     [Google Scholar]
  33. Wolk, C. P. (1996); Heterocyst formation.. Annu Rev Genet, 30,:59–78 1993
    [Google Scholar]
  34. de Zamaroczy, M., Paquelin, A., Elmerich, C. (1993); Functional organization of the glnB—glnA cluster of Azospirillum brasilense.. J Bacteriol, 175,:2507–2515 1993
    [Google Scholar]
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Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133: glnB or the PII protein may be essential
Microbiology 144, 1537 (1998); https://doi.org/10.1099/00221287-144-6-1537
/content/journal/micro/10.1099/00221287-144-6-1537
/content/journal/micro/10.1099/00221287-144-6-1537
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