1887

Abstract

The motile TnphoA mutant IC24 of Proteus mirabilis U6450 generates an aberrant swarming colony, and was shown to be impaired in swarm cell differentiation, i.e. cell elongation and hyperflagellation, causing delayed and slower population migration across a solid growth medium. Levels of transcript from the flagellin filament gene fliC, the flagellar master operon flhDC, and the leucine-responsive regulatory protein gene Irp, a regulator of swarming differentiation, were reduced in IC24 mutant swarm cells. The transposon had inserted into a gene encoding a putative P-type ATPase closely related to those transporting cations across bacterial membranes. This ppa gene (Proteus P-type ATPase) was maximally expressed in differentiated swarm cells. The data suggest an effect of ion homeostasis on swarm cell differentiation, possibly mediated via the Irp--flhDC pathway.

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1998-07-01
2024-04-19
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References

  1. Allison C., Hughes C. 1991a; Bacterial swarming: an example of prokaryotic differentiation and multicellular behaviour.. Sci Prog 75:403–422
    [Google Scholar]
  2. Allison C., Hughes C. 1991b; Closely linked genetic loci required for swarm cell differentiation and multicellular migration by Proteus mirabilis. . Mol Microbiol 5:1975–1982
    [Google Scholar]
  3. Allison G, Lai H.C-., Hughes C. 1992; Co-ordinate expression of virulence genes during swarm-cell differentiation and population migration of Proteus mirabilis. . Mol Microbiol 6:1583–1591
    [Google Scholar]
  4. Allison G, Lai H.C-., Gygi D., Hughes C. 1993; Cell differentiation of Proteus mirabilis is initiated by glutamine, a specific chemoattractant for swarming cells.. Mol Microbiol 8:53–60
    [Google Scholar]
  5. Allison G., Ernody L., Coleman N., Hughes C. 1994; The role of swarm cell differentiation and multicellular migration in the uropathogenicity of Proteus mirabilis.. J Infect Dis 169:1155–1158
    [Google Scholar]
  6. Beard S.J., Hashim R., Membrillo-Hernandez J., Hughes M.N., Poole R.K. 1997; Zinc (II) tolerance in Escherichia coli K12: evidence that the zntA gene (o732) encodes a cation transport ATPase.. Mol Microbiol 25:883–891
    [Google Scholar]
  7. Belas R. 1994; Expression of multiple flagellin-encoding genes from Proteus mirabilis. . J Bacteriol 176:7169–7181
    [Google Scholar]
  8. Belas R., Flaherty D. 1994; Sequence and genetic analysis of multiple flagellin-encoding genes from Proteus mirabilis. . Gene 128:33–41
    [Google Scholar]
  9. Belas R., Erskine D., Flaherty D. 1991; Proteus mirabilis mutants defective in swarmer cell differentiation and multicellular behaviour.. J Bacteriol 173:6279–6288
    [Google Scholar]
  10. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. . Nucleic Acids Res 12:387–395
    [Google Scholar]
  11. Eberl L., Christiansen G., Molin S., Givskov M.L. 1996; Differentiation of Serratia liquefaciens into swarm cells is controlled by the expression of the flhD master operon.. J Bacteriol 178:554–559
    [Google Scholar]
  12. Furness R.B., Fraser G.M., Hay N.A., Hughes C. 1997; Negative feedback from a Proteus Class II flagellum export defect to the flhDC master operon controlling cell division and flagellum assembly.. J Bacteriol 179:5585–5588
    [Google Scholar]
  13. Gaisser S., Hughes C. 1997; A locus coding for putative non- ribosomal peptide/polyketide synthase functions is mutated in a swarming-defective Proteus mirabilis strain.. Mol Gen Genet 253:415–427
    [Google Scholar]
  14. Gilbert J.V., Ramakrishna J., Sunderman F.W. Jr Wright A., Plaut A.G. 1995; Protein Hpn: cloning and characterization of a histidine-rich metal-binding polypeptide in Helicobacter pylori and Helicobacter mustelae. . Infect Immun 63:2682–2688
    [Google Scholar]
  15. Gygi D., Bailey M. J., Allison C., Hughes C. 1995a; Requirement for FlhA in flagella assembly and swarm cell differentiation by Proteus mirabilis. . Mol Microbiol 15:761–769
    [Google Scholar]
  16. Gygi D., Rahman M. M., Lai H.C-., Carlson R., Guard-Petter J., Hughes C. 1995b; A cell-surface polysaccharide that facilitates rapid population migration by differentiated swarm cells of Proteus mirabilis. . Mol Microbiol 17:1167–1175
    [Google Scholar]
  17. Gygi D., Fraser G., Dufour A., Hughes C. 1997; A motile but non-swarming mutant of Proteus mirabilis lacks FlgN, a facilitator of flagella filament assembly.. Mol Microbiol 25:597–604
    [Google Scholar]
  18. Hay N.A., Tipper J.T., Gygi D., Hughes C. 1997; A nonswarming mutant of Proteus mirabilis lacks the Lrp global transcriptional regulator.. J Bacteriol 179:4741–4746
    [Google Scholar]
  19. Kyte J., Doolittle R.F. 1982; A simple method for displaying the hydropathic character of a protein.. J Mol Biol 157:105–132
    [Google Scholar]
  20. Melton D.A., Krieg P.A., Rebagliati M.R., Maniatis T., Zinn K., Green M.R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.. Nucleic Acids Res 12:7035–7056
    [Google Scholar]
  21. Nucifora G., Chu L., Misra T.K., Silver S. 1989; Cadmium resistance from Staphylococcus aureus plasmid pI258 CadA gene results from a cadmium-efflux ATPase.. Proc Natl Acad Sci USA 863544–3548
    [Google Scholar]
  22. Odermatt A., Suter H., Krapf R., Solioz M. 1993; Primary structure of 2 P-type ATPases involved in copper homeostasis in Enterococcus hirae. . J Biol Chern 268:12775–12779
    [Google Scholar]
  23. Rensing C., Bhattati M., Rosen B.P. 1997; The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase.. Proc Natl Acad Sci USA 94:14326–14331
    [Google Scholar]
  24. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Shi N.Y., Li GY., Louise GJ., Adler J. 1993; Mechanism of adverse conditions causing lack of flagella in Escherichia coli. . J Bacteriol 175:2236–2240
    [Google Scholar]
  26. Silver S., Walderhaug G. 1992; Gene regulation of plasmid- and chromosome-determined inorganic ion transport in bacteria.. Microbiol Rev 56:195–228
    [Google Scholar]
  27. Sofia H.J., Burland V., Daniels D.L., Plunkett G. III Blattner F.R. 1994; Analysis of the Escherichia coli. DNA sequence of the region from 76·0-minutes to 81·5 minutes.. Nucleic Acids Res 22:2576–2586
    [Google Scholar]
  28. Tisa L.S., Adler J. 1992; Calcium ions are involved in Escherichia coli chemotaxis.. Proc Natl Acad Sci USA 8911804–11808
    [Google Scholar]
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