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Volume 145, Issue 11

PepR1, a CcpA-like transcription regulator of subsp. Free

Abstract

The PepR1 protein from subsp. DSM 7290 shares extensive homology with catabolite-control proteins from various Gram-positive bacteria. Expression of the subcloned gene allowed for partial complementation of a defect in . The influence of PepR1 on transcription of the prolidase gene , which is located adjacent to , was examined by use of reporter gene fusions in . PepR1 stimulated transcription initiation at the promoter about twofold, and this effect required the integrity of a 14 bp palindromic -like sequence located 74 nt upstream of . In gel-mobility-shift assays, PepR1 specifically interacted with the promoter region and also with DNA fragments covering the promoters of the , and genes of subsp. , which encode two additional peptidases and a branched-chain amino acid transporter, respectively. -like elements were identified in each of these DNA fragments. Catabolite control of PepQ was demonstrated in subsp. . During growth with lactose the enzyme activity was twofold higher than in the presence of glucose, and corresponding differences were also detected in the level of transcription.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): catabolite control , cre sequence , Lactobacillus delbrueckii , PepR1 transcriptional regulator and peptidase Q
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1999-11-01
2024-04-19
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PepR1, a CcpA-like transcription regulator of Lactobacillusdelbrueckii subsp. lactis
Microbiology 145, 3147 (1999); https://doi.org/10.1099/00221287-145-11-3147
/content/journal/micro/10.1099/00221287-145-11-3147
/content/journal/micro/10.1099/00221287-145-11-3147
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