1887

Abstract

A multicopper oxidase gene from the human pathogenic yeast was isolated and characterized. An open reading frame of 1872 bp, designated , was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 705 kDa. The identity between the deduced amino acid sequences of and the gene is 55%. was localized on chromosome 6. A null mutant (Δ/Δ) was constructed by sequential gene disruption. Unlike the SC5314 wild-type strain the Δ mutant was unable to grow in low-iron medium. The lack of growth of a Δ mutant in iron-limited medium was compensated by transformation with . The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.

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1999-09-01
2024-03-29
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipmann D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Askwith C., Eide D., Van Ho A. V., Bernard P. S., Li L., Davis-Kaplan S., Sipe D. M., Kaplan J. 1994; The FET3 gene of Saccharomyces cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell 76:403–410 [CrossRef]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1995 Current Protocols in Molecular Biology New York: Wiley;
    [Google Scholar]
  4. Boeke J. D., LaCroute F., Fink G. R. 1984; A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197:345–346 [CrossRef]
    [Google Scholar]
  5. Borg-von Zepelin M., Wagner T. 1995; Fluorescence assay for the detection of adherent Candida yeasts to target cells in microtest plates. Mycoses 38:339–347 [CrossRef]
    [Google Scholar]
  6. Dancis A., Yuan D. S., Haile D., Askwith C., Eide D., Moehle C., Kaplan J., Klausner R. D. 1994; Molecular characterization of a copper transport protein in Saccharomyces cerevisiae: an unexpected role for copper in iron transport. Cell 76:393–402 [CrossRef]
    [Google Scholar]
  7. De Silva D. M., Askwith C. C., Eide D., Kaplan J. 1995; The FET3 gene product required for high affinity iron transport in yeast is a cell surface ferroxidase. J Biol Chem 270:1098–1101 [CrossRef]
    [Google Scholar]
  8. Dix D., Bridgham J., Broderius M., Eide D. 1997; Characterization of the FET4 protein of yeast. J Biol Chem 272:11770–11777 [CrossRef]
    [Google Scholar]
  9. Eck R., Bergmann C., Ziegelbauer K., Schönfeld W., Künkel W. 1997; A neutral trehalase gene from Candida albicans: molecular cloning, characterization and disruption. Microbiology 143:3747–3756 [CrossRef]
    [Google Scholar]
  10. Eide D., Guarente L. 1992; Increased dosage of a transcriptional activator gene enhances iron-limited growth of Saccharomyces cerevisiae. J Gen Microbiol 138:347–354 [CrossRef]
    [Google Scholar]
  11. Eide D., Davis-Kaplan S., Jordan I., Sipe D., Kaplan J. 1992; Regulation of iron uptake in Saccharomyces cerevisiae.. J Biol Chem 267:20774–20781
    [Google Scholar]
  12. Fonzi W. A., Irvine M. Y. 1993; Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717–728
    [Google Scholar]
  13. Fratti R. A., Belanger P. H., Ghannoum M. A., Edwards J. E.Jr, Filler S. G. 1998; Endothelial cell injury caused by Candida albicans is dependent on iron. Infect Immun 66:191–196
    [Google Scholar]
  14. Georgatsou E., Alexandraki D. 1994; Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 14:3065–3073
    [Google Scholar]
  15. von Heijne G. 1983; Pattern of amino acids near signal-sequence cleavage sites. Eur J Biochem 133:17–21 [CrossRef]
    [Google Scholar]
  16. Henikoff S., Henikoff J. G. 1994; Protein family classification based on searching a database of blocks. Genomics 19:97–107 [CrossRef]
    [Google Scholar]
  17. Holzberg M., Artis W. M. 1983; Hydroxamate siderophore production by opportunistic and systemic fungal pathogens. Infect Immun 40:1134–1139
    [Google Scholar]
  18. Ismail A., Bedell G. W., Lupan D. M. 1985; Siderophore production by the pathogenic yeast, Candida albicans. Biochem Biophys Res Commun 130:885–891 [CrossRef]
    [Google Scholar]
  19. Klebe I. K., Harris I. V., Sharp D., Douglas M. G. 1983; A general method for polyethylenglycol-induced genetic transformation of bacteria and yeast. Gene 25:333–341 [CrossRef]
    [Google Scholar]
  20. Lloyd A. T., Sharp P. M. 1992; Evolution of codon usage patterns: the extent and nature of divergence between Candida albicans and Saccharomyces cerevisiae. Nucleic Acids Res 20:5289–5295 [CrossRef]
    [Google Scholar]
  21. Morrissey J. A., Williams P. H., Cashmore A. M. 1996; Candida albicans has a cell-associated ferric-reductase activity which is regulated in response to levels of iron and copper. Microbiology 142:485–492 [CrossRef]
    [Google Scholar]
  22. Morrow B. E., Ju Q., Warner J. R. . 1993; A bipartic DNA-binding domain in yeast Reb1p. Mol Cell Biol 13:1173–1182
    [Google Scholar]
  23. Plempel M. 1984; Antimycotic activity of Bay N 7133 in animal experiments. J Antimicrob Chemother 13:447–463 [CrossRef]
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Spizzo T., Byersdorfer C., Duesterhoeft S., Eide D. 1997; The yeast FET5 gene encodes a FET3-related multicopper oxidase implicated in iron transport. Mol Gen Genet 256:547–556
    [Google Scholar]
  26. Stearman R., Yuan D. S., Yamaguchi-Iwai Y., Klausner R. D., Dancis A. 1996; A permease–oxidase complex involved in high-affinity iron uptake in yeast. Science 271:1552–1557 [CrossRef]
    [Google Scholar]
  27. Sweet S. P., Douglas L. J. 1991; Effect of iron concentration on siderophore synthesis and pigment production by Candida albicans. FEMS Microbiol Lett 80:87–92 [CrossRef]
    [Google Scholar]
  28. Swoboda R. K., Bertram G., Budge S., Gow N. A. R., Gooday W., Brown A. J. P. 1995; Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans. Infect Immun 63:4506–4514
    [Google Scholar]
  29. Yamaguchi-Iwai Y, Dancis A., Klausner R. D. 1995; AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae. EMBO J 14:1231–1239
    [Google Scholar]
  30. Yuan D. S., Stearman R., Dancis A., Dunn T., Beeler T., Klausner R. D. 1995; The Menkes/Wilson disease gene homologue in yeast provides copper to a ceruloplasmin-like oxidase required for iron uptake. Proc Natl Acad Sci USA 92:2632–2636 [CrossRef]
    [Google Scholar]
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