1887

Abstract

The antifungal metabolite 2,4-diacetylphloroglucinol plays a major role in the biocontrol capabilities of . The phloroglucinol biosynthetic locus of F113 has been isolated previously. From nucleotide sequence data, a putative regulator gene () was identified upstream and divergently transcribed from the phloroglucinol biosynthetic genes. PhlF shows similarity to various transcriptional repressors in the EMBL database and exhibits a helix–turn–helix motif in its amino acid sequence. was cloned into an expression vector and the PhlF protein product was purified. Gel retardation experiments demonstrated PhlF to be a DNA-binding protein and showed that it binds to the intergenic region. Introduction of into F113 in multiple copies resulted in repression of phloroglucinol production in this strain. This effect was mediated at the transcription level since the expression of a phloroglucinol biosynthetic gene fusion in this background was equally repressed. Furthermore, the inactivation of results in derepression of phloroglucinol production in this strain.

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2000-02-01
2024-03-28
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