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Volume 147, Issue 10

Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Free

Abstract

Listeriolysin O (LLO) is a major virulence factor secreted by the pathogenic and acts as pore-forming cytolysin. Based on sequence similarities between LLO and perfringolysin (PFO), the cytolysin from of known crystallographic structure, two truncated LLO proteins were produced: LLO-d123, comprising the first three predicted domains, and LLO-d4, the last C-terminal domain. The two proteins were efficiently secreted into the culture supernatant of and were able to bind to cell membranes. Strikingly, when expressed simultaneously, the two secreted domains LLO-d123 and LLO-d4 reassembled into a haemolytically active form. Two in-frame linker insertions were generated in the hinge region between the d123 and d4 domains. In both cases, the insertion created a major cleavage site for proteolytic degradation and abolished cytolytic activity, which might suggest that the region connecting d123 and d4 participates in the interaction between the two portions of the monomer.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): domain interactions , HRBC, horse red blood cells , linker insertions , LLO mutants , LLO, listeriolysin O , PFO, perfringolysin and truncated proteins
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2001-10-01
2024-04-18
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Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Listeria monocytogenes
Microbiology 147, 2679 (2001); https://doi.org/10.1099/00221287-147-10-2679
/content/journal/micro/10.1099/00221287-147-10-2679
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